フルテキストURL HP110_5_2_S81.pdf Fig.pdf Table.pdf
著者 Yumoto, Yasuhiro| Okada, Shigeru| Kinno, Ikuo| Nagamatsu, Tomohiro| Nouso, Kazuhiro| Nakayama, Eiichi|
キーワード operational topics waste low-level waste disposal waste management
発行日 2016-05
出版物タイトル Health Physics
110巻
5 S2号
出版者 LWW
開始ページ S81
終了ページ S87
ISSN 0017-9078
NCID AA00662510
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン author
PubMed ID 27023155
DOI 10.1097/HP.0b013e318254aef6
Web of Science KeyUT 000373920200006
関連URL isVersionOf https://doi.org/10.1097/HP.0b013e318254aef6
JaLCDOI 10.18926/AMO/55589
フルテキストURL 71_6_519.pdf
著者 Mizukawa, Nobuyoshi| Swe Swe Win| Zaw Moe Thein| Moe Thida Htwe| Yoshioka, Yohsuke| Kimata, Yoshihiro| Iida, Seiji| Khin, Myo| Okada, Shigeru| Sein, Than|
抄録 Oral cancer is a very common disease in South and Southeast Asia. Betel quid (BQ)- chewing and tobaccosmoking habits are etiological factors for oral cancer patients in these regions. We conducted an oral cancer screening in BQ-chewing endemic rural areas in South Myanmar for the early detection of oral cancer in BQ-chewing and smoking individuals. We examined 105 subjects who were at high risk of oral cancer due to their oral habits (BQ users and/or smokers). Three carcinoma cases were detected, and there were 8 dysplasia cases. The carcinoma detection rate was 2.9%, and the carcinoma and precancerous lesion detection rate was 10.5%. In Myanmar, oral cancer screening has been conducted sporadically on a voluntary basis, and nationwide surveys have never been performed. There are also few reports of oral cancer screening for high-risk groups among the general population in Myanmar. Our present findings highlight the need for further screening and surveys. Education on betel quid chewing- and tobacco- related oral diseases and screening for the early detection of oral cancer are of the utmost importance in the control and prevention of oral cancer.
キーワード oral cancer screening betel quid (BQ) Myanmar oral cancer smoking
Amo Type Original Article
発行日 2017-12
出版物タイトル Acta Medica Okayama
71巻
6号
出版者 Okayama University Medical School
開始ページ 519
終了ページ 524
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2017 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 29276225
JaLCDOI 10.18926/AMO/53122
フルテキストURL 69_1_51.pdf
著者 Mu Mu Shwe| Kyi Kyi Nyunt| Okada, Shigeru| Harano, Teruo| Hlaing Myat Thu| Hla Myat Mo Mo| Mo Mo Win| Khin Khin Oo| KhinThet Wai| Khin Saw Aye| Myo Khin|
抄録 Persistent infection with oncogenic types of human papillomavirus (HPV) is the most important risk factor associated with cervical cancer. This study detected the oncogenic HPV genotypes in cervical neoplasia in relation to clinicopathological findings using a cross-sectional descriptive method in 2011 and 2012. Cervical swabs and colposcopy-directed cervical biopsy tissues were collected from 108 women (median age 45 years;range 20-78) showing cervical cytological changes at Sanpya General Hospital, Yangon, Myanmar. HPV DNA testing and genotyping were performed by polymerase chain reaction and restriction fragment length polymorphism. HPV was identified in women with cervical intraepithelial neoplasia (CIN) 1 (44.4%), CIN2 (63.2%), CIN3 (70.6%), and squamous cell carcinoma (SCC) (74.1%). The association between cervical neoplasia and HPV positivity was highly significant (p=0.008). Most patients infected with HPV were between 40-49 years of age, and the youngest were in the 20- to 29-year-old age group. The most common genotype was HPV 16 (65.6%) with the following distribution:70% in CIN1, 41.7% in CIN2, 91.7% in CIN3, and 60% in SCC. HPV-31 was the second-most frequent (21.9%):30% in CIN1, 33.3% in CIN2, 8.3% in CIN3, and 15% in SCC. The third-most frequent-genotype was HPV-18 (7.8%):8.3% in CIN1, and 20% in SCC. Another genotype was HPV-58 (4.7%):16.7% in CIN1 and 5% in SCC. The majority of CIN/SCC cases were associated with HPV genotypes 16, 31, 18, and 58. If oncogenic HPV genotypes are positive, the possibility of cervical neoplasia can be predicted. Knowledge of the HPV genotypes distribution can predict the effectiveness of the currently used HPV vaccine.
キーワード human papillomavirus genotyping Myanmar
Amo Type Original Article
発行日 2015-02
出版物タイトル Acta Medica Okayama
69巻
1号
出版者 Okayama University Medical School
開始ページ 51
終了ページ 58
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 25703171
Web of Sience KeyUT 000349740300006
JaLCDOI 10.18926/AMO/52404
フルテキストURL 68_2_79.pdf
著者 Mu-Mu-Shwe| Harano, Teruo| Okada, Shigeru| Aye-Aye-Win| Khin-Saw-Aye| Hlaing-Myat-Thu| Mo-Mo-Win| Khin-Khin-Oo| Myo-Khin|
抄録 This study aimed to determine the prevalence of normal and abnormal cervical cytology in women who attended the cervical cancer screening clinic of the Department of Medical Research in Lower Myanmar, and to determine the proportion of high-risk (HR) human papillomavirus (HPV) infection and HPV genotypes in women with normal and abnormal cervical cytology. A total of 1,771 women were screened from 2010 to 2011. Among them, 762 women (43.0%) had a normal smear, and 866 (48.9%) and 87 (4.9%) were diagnosed with inflammatory smears and atypical squamous cells of undetermined significance (ASCUS), respectively. Diagnoses of low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL) numbered 42 (2.3%) and 11 (0.6%) respectively. Three cases of squamous cell carcinoma (SCC) (0.2%) were detected. Cervical swabs were collected from 96 women with abnormal cervical cytology and 20 with normal cytology. HR-HPV DNA testing was performed by polymerase chain reaction (PCR) with pU1M/pU2R primers. HR-HPV were identified in 35.5% (22/62) of inflammatory smears, 60% (6/10) of ASCUS, 86.7% (13/15) of LSIL, 50% (3/6) of HSIL, 100% (3/3) of SCC and 5% (1/20) of normal cytology. In PCR-positive cases, HPV genotyping was analyzed by the cleaved amplification polymorphism method. The most prevalent HPV genotypes were HPV-16 (60.4%) followed by HPV-31 (14.6%), HPV-18 (12.5%) and HPV-58 (12.5%). Women with abnormal cervical cytology were 10 times more likely to be HR-HPV positive than those with normal cytology (p=0.0001). This study suggests that the implementation of a cervical cytology screening program and routine vaccination against HPV in preadolescent and adolescent groups are needed to reduce the burden of HPV-associated cervical cancer.
キーワード human papillomavirus cervical neoplasia genotyping Myanmar
Amo Type Original Article
発行日 2014-04
出版物タイトル Acta Medica Okayama
68巻
2号
出版者 Okayama University Medical School
開始ページ 79
終了ページ 87
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2014 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 24743783
Web of Science KeyUT 000334652700003
JaLCDOI 10.18926/AMO/40507
フルテキストURL 64_5_317.pdf
著者 Myo-Khin| San-San-Oo| Khin, May Oo| Shimono, Kunio| Koide, Norio| Okada, Shigeru|
抄録 We studied the prevalence of hepatitis C virus infection among blood donors from 3 hospitals of Central Myanmar and 7 hospitals of Lower Myanmar in the Yangon area, and analyzed the factors associated with the infection. The study period was from November, 2005 to June, 2007. A pre-tested questionnaire was used to obtain information on age, ethnic group, marital status, tattooing, body piercing, history of receiving transfusions, and liver diseases in self and in sexual partners. Data on seropositivity to hepatitis C, hepatitis B and human immunodeficiency virus infections were recorded. A total of 65,240 blood donors participated in the study. Their ages ranged from 18 years to 60 years (mean±SD=29.5±9.3). The male-to-female ratio was 6:1. The prevalence of the antibody to hepatitis C was found to be 0.95% with varying rates (0.34 to 2.03) among hospitals. Females had a slightly higher rate (1.06%) than males (0.93%) (p=0.237). Multivariate analyses revealed the following factors to be related to HCV infection:HIV infection, odds ratio (OR)=3.0 (p=0.003);history of liver disease, OR=8.9 (p=0.001);and age 30 years and above, OR=2.6 (p=0.001). We discuss the varying prevalences of HCV around the world.
キーワード Myanmar hepatitis C prevalence blood donors associated factors
Amo Type Original Article
発行日 2010-10
出版物タイトル Acta Medica Okayama
64巻
5号
出版者 Okayama University Medical School
開始ページ 317
終了ページ 321
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2010 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 20975765
Web of Science KeyUT 000283563300007
JaLCDOI 10.18926/AMO/40132
フルテキストURL 64_4_243.pdf
著者 Myo-Khin| Myat-Tin-Htwe-Kyaw| Yi-Yi-Kyaw| Ohmar-Lwin| Myat-Phone-Kyaw| Khin-May-Oo| Shimono, Kunio| Koide, Norio| Okada, Shigeru|
抄録 The efficacy of AntiOxidant Biofactor (AOB(R)) for the management of apparently healthy subjects with chronic hepatitis C infection was investigated. A total of 60 subjects (35 males, 25 females) participated in the trial. AOB was given orally in 2 packs (3g per pack) 3 times per day. 17 subjects had taken AOB for 3 years, 31 subjects up to 2 years, and 41 subjects up to one year. The initial mean (SD) serum alamine aminotransferase (ALT) level was 46.3+/-35.4IU/L, and significant (p0.05, paired t-test) reductions in the mean serum ALT levels were observed at 6 months (38.6+/-21.5IU/L), 18 months (31.9+/-18.1IU/L), 2 years (31.2+/-14.6IU/L), and 3 years (28.0+/-15.9IU/L). Those presenting with high serum ALT levels (30 subjects) demonstrated significant levels (p0.05, paired t-test) of reduction in the mean serum ALT levels at 6, 12, 18, 24, and 36 months of treatment. No side effects were observed and the AOB treatment was well tolerated by all subjects.
キーワード hepatitis C AntiOxidant Biofactor (AOB®) ALT level
Amo Type Original Article
発行日 2010-08
出版物タイトル Acta Medica Okayama
64巻
4号
出版者 Okayama University Medical School
開始ページ 243
終了ページ 248
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 20802541
Web of Science KeyUT 000281384400005
JaLCDOI 10.18926/AMO/32811
フルテキストURL fulltext.pdf
著者 Fujisawa, Masayoshi| Notohara, Kenji| Tsukayama, Choutatsu| Mizuno, Ryuichirou| Okada, Shigeru|
抄録 <p>We observed the distribution of CD56+ epithelial cells in the pancreatic duct system using 25 fetal, one infantile, 3 normal adult, 4 diabetic, and 8 chronically inflamed pancreatic tissue samples. In the early stage of gestation (12 to 17 weeks), CD56+ cells were commonly seen in the immature tubular structures. They were often continuous to pancreatic islets, and their distribution was similar to that of synaptophysin (Syn)+ cells, suggesting that they are precursors of islet neogenesis. Their number decreased in proportion to gestational age. Instead, from 24 weeks of gestation, luminal cell clusters that were common in interlobular ducts revealed CD56+. These cell clusters were unrelated to islet neogenesis and Syn expression. Similar CD56+ luminal cell clusters were also observed in cases of chronic pancreatitis, whereas they were scarce in normal adult and diabetic tissues. CD56+ cells were also occasionally seen in intralobular ducts, intercalated ducts, and centroacinar cells in cases of chronic pancreatitis. We conclude that there are two types of CD56+ epithelial cells in the pancreatic duct system: CD56+ endocrine cells are numerous during the early stage of gestation, when islet neogenesis appears, while CD56+ luminal cells may represent developmental and regenerative changes of pancreatic ducts.</p>
キーワード CD56 pancreas development pancreatitis islets
Amo Type Article
発行日 2003-12
出版物タイトル Acta Medica Okayama
57巻
6号
出版者 Okayama University Medical School
開始ページ 279
終了ページ 284
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 14726964
Web of Sience KeyUT 000187556500002
JaLCDOI 10.18926/AMO/32701
フルテキストURL fulltext.pdf
著者 Okada, Shigeru| Okamoto, Atsuko| Awai, Michiyasu| Naito, Mitsuko| Seno, Satimaru|
抄録 <p>To observe the possible role of cAMP on the DNA synthesis during specialization-division of myelogenous precursor cells, the authors observed the DNA and RNA synthesis of the cells by in vitro autoradiography. And it is concluded that cAMP or its dibutyryl derivative added to the media penetrated into myelogenous precursor cells and metamyelocytes of mice and enhanced the DNA synthetic capacity of them. cAMP hardly enhanced RNA synthesis. Discussion is made on relation between enhancement of DNA synthesis of metamyelocytes and their possible rejuvenation.</p>
Amo Type Article
発行日 1974-04
出版物タイトル Acta Medica Okayama
28巻
2号
出版者 Okayama University Medical School
開始ページ 81
終了ページ 87
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4368682
NAID 120002311417
JaLCDOI 10.18926/AMO/32625
フルテキストURL fulltext.pdf
著者 Yu, Ying-yan| Ogino, Teteuya| Okada, Shigeru|
抄録 <p>Constitutional lipid peroxidation in randomly selected 32 cases of clinically advanced carcinoma from human gastrointestinal tract (20 cases), breast (8 cases) and kidney (4 cases) was examined histochemically in frozen sections using cold Schiff's reagent. Only two cases of gastrointestinal carcinoma were positive by the reagent. Non-cancerous parenchymal cells were negative. These findings suggest that detectable constitutional lipid peroxidation seldom occurs in either cancerous or normal tissues. The capacity for normal and neoplastic tissues to undergo lipid peroxidation was also studied by incubation with an iron-NADPH pro-oxidant system. Normal parenchymal cells showed, to various degrees, a positive reactivity. In gastrointestinal carcinoma, 6 out of 7 cases of well differentiated adenocarcinoma reacted positively, whereas 2 out of 8 cases of moderately to poorly differentiated adenocarcinoma disclosed weakly positive reactions. Mucinous adenocarcinomas (4 cases) were all negative. Signet-ring cell carcinoma (1 case) was positive. One out of 8 cases of breast cancer also showed positive reaction. Four renal cell carcinomas were all negative. Cancer cells have lower capacity to undergo lipid peroxidation than normal cells, when the iron-NADPH pro-oxidant system was employed. In gastrointestinal carcinoma, the ability to undergo lipid peroxidation by the iron-NADPH pro-oxidant seems to be correlated with their histological differentiation. This fact may suggest that differences in lipid composition or the NADPH enzyme system exist between well differentiated and poorly differentiated gastrointestinal malignancies.</p>
キーワード lipid peroxidation histochemistry cancer iron NADPH
Amo Type Article
発行日 1992-08
出版物タイトル Acta Medica Okayama
46巻
4号
出版者 Okayama University Medical School
開始ページ 233
終了ページ 239
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1442147
Web of Science KeyUT A1992JL44200002
JaLCDOI 10.18926/AMO/32554
フルテキストURL fulltext.pdf
著者 Okada, Shigeru|
抄録 <p>For the purpose of revealing whether the sensitivity of the erythropoiesis to actinomycin D (AMD) differs among different animal species, and to see the acting site of AMD on erythroid cell specialization stage, the author observed the hourly change of the blood cell counts and bone marrow cells after AMD administration to mice, rats and rabbits, and obtained the following results: 1. The data indicated that the erythropoiesis of ra bbit is sensitive to AMD, as much as that of mice, while the rat is resistant to AMD, and its erythropoiesis is not affected by the similar dose of AMD as in the case of mouse and rabbit. 2. The morphologic observations on the eradication process of erythroblasts in the bone marrow of mice and rabbits indicates that AMD acts as to inhibit the transformation of the stem cell to the proerythroblast but not on the erythroblast in the course of specialization. The time required for the eradication coincided with the time of the proerythroblast to the mature red cell. 3. Discussion has been made on the possibility of the common stem cell to erythroid and granulocytic cells in relation to the lymphoid cells in bone marrow and their blast form.</p>
Amo Type Article
発行日 1969-06
出版物タイトル Acta Medicinae Okayama
23巻
3号
出版者 Okayama University Medical School
開始ページ 165
終了ページ 176
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4242841
NAID 120002311439
JaLCDOI 10.18926/AMO/32545
フルテキストURL fulltext.pdf
著者 Okada, Shigeru|
抄録 <p>For the purpose of revealing whether or not hemoglobin synthesis is inhibited by the AMD, the author estimated the hemoglobin level of AMD treated anilmals by microspectrophotometer, and found that the hemoglobin levels of all the developmental stages of erythroid cells were not inhibited by the AMD. The data indicated that about one half of mRNA for hemoglobin is synthesized in the early stage of specialization with the supplementary synthesis at the later stages and all these mRNA is stable and insensitive to AMD.</p>
Amo Type Article
発行日 1969-10
出版物タイトル Acta Medicinae Okayama
23巻
5号
出版者 Okayama University Medical School
開始ページ 387
終了ページ 392
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4244047
NAID 120002312271
JaLCDOI 10.18926/AMO/32515
フルテキストURL fulltext.pdf
著者 Okada, Shigeru|
抄録 <p>For the purpose of revealing whether AMD inhibits the RNA synthesis of erythroblasts in an effective dose in vivo to eradicate erythroid cells in rabbit bone marrow, the author observed the RNA synthesis by H3-uridine incorporation in vitro and RNA level on the cells from the anemic animals taken at a certain period after a single injection of AMD in a small dose of 50 and 100&#956;g/kg body weight. The data revealed that by such a small dose of injection of AMD the RNA synthesis of erythroid precursors, early basophilic and proerythroblast stages, was successfully suppressed without any suppressing effect on the RNA synthesis of erythroblasts in the later stages of specialization, indicating that there are at least two kinds of RNA synthesis: one seen mainly in the earlier stages of specialization and the other one seen mainly in the later stages, and they can be distinguished from each other by the AMD sensitivity.</p>
Amo Type Article
発行日 1969-08
出版物タイトル Acta Medicinae Okayama
23巻
4号
出版者 Okayama University Medical School
開始ページ 265
終了ページ 271
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4243559
NAID 120002311801
JaLCDOI 10.18926/AMO/32499
フルテキストURL fulltext.pdf
著者 Kimoto, Tetsuo| Shigehisa, Morio| Hayashi, Kenj| Okada, Shigeru| Sogabe, Koichi| Grace, James T.|
抄録 <p>In the immunofluorescent study it has been revealed that rabbit sera immunized with transformed cells induced by SV-40 DNA, produce circulating antibody capable of re:lcting with intranuclear antigens synthesized by SV-40 complyte virus transforming process, In addition, the result confirmed that SV-40 DNA replicates DNA-containing viruses in the host cell and that also the genome coding for the synthesis of SV-40 tumor antigen is resposible for viral DNA.</p>
Amo Type Article
発行日 1967-04
出版物タイトル Acta Medicinae Okayama
21巻
2号
出版者 Okayama University Medical School
開始ページ 51
終了ページ 57
NCID AA00041342
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 4294709
NAID 120002312000
JaLCDOI 10.18926/AMO/32299
フルテキストURL fulltext.pdf
著者 Okada, Shigeru| Taketa, Kazuhisa| Ishikawa, Takatoshi| Koji, Takehiko| Swe, Than| Win, Ne| Win, Khin Maung| Mra, Rai| Myint, Thein Thein|
抄録 <p>We conducted Myanmar-Japan cooperation studies on hepatitis B and hepatitis C virus markers in patients with thalassemias and those with liver diseases. Among the 102 patients with liver diseases, 92% had a history of hepatitis B virus infection (antibody to hepatitis B core antigen positive), 35% were hepatitis B surface antigen positive, 39% were positive for anti-HCV. Among 28 patients with hepatocellular carcinoma, 46% had hepatitis B surface antigen, 21.4% had antibody to hepatitis C virus, and 7% were positive for both hepatitis B surface antigen and anti hepatitis C virus. The history of HCV infection among blood recipients at the Haematology Department of the Yangon General Hospital and at the Yangon Children's Hospital was found to be 55.5% and 46.7%, respectively, which is comparable to the history of hepatitis B infection (66.7% and 46.7%, respectively). This preliminary survey also encountered 2 cases positive for anti-HCV among 34 voluntary blood donors. This survey is the first one to report that hepatitis C is at the epidemic stage in Myanmar. As there is no effective treatment for hepatitis C in this country, a screening program for blood used in transfusion should be started immediately.</p>
キーワード hepatitis C Myanmar (Burma) thalassemia hepatitis B
Amo Type Article
発行日 2000-06
出版物タイトル Acta Medica Okayama
54巻
3号
出版者 Okayama University Medical School
開始ページ 137
終了ページ 138
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 10925739
Web of Science KeyUT 000087965700007
JaLCDOI 10.18926/AMO/32180
フルテキストURL fulltext.pdf
著者 Liu, Miao| Okada, Shigeru| Kawabata, Teruyuki|
抄録 <p>&lt;p&gt;Iron plays a critical role in the production of activated oxygen species and the activity of chelated iron in the biological system depends on the chemical forms of the chelators. In the present study, we used ferric nitrolotriacetate (Fe-NTA, molar ratio of iron to chelators = 1:3), ferric ethylenediaminetetraacetate (Fe-EDTA, 1:3 complex) and ferric Desferal (Fe-Des, 1:1.1 complex) to see their &quot;free&quot; iron content in aqueous solutions in vitro and in the serum obtained after a single intraperitoneal injection of the chelates to rats (7.5 mg of iron/kg). &quot;Free&quot; iron was measured by the bleomycin-assay system. When Fe-NTA was dissolved in water, &quot;free&quot; iron increased linearly with total iron concentration up to 10 microM, whereas Fe-EDTA and Fe-Des showed no &quot;free&quot; iron with corresponding iron concentrations. When these three ferric chelates were dissolved in normal rat serum, &quot;free&quot; iron in Fe-NTA increased abruptly between 40 microM and 60 microM iron concentrations, then increased slowly up to 100 microM. Fe-Des did not show any &quot;free&quot; iron at comparable iron concentrations. Fe-EDTA had an intermediate &quot;free&quot; iron level in the serum. Among the ferric chelate complexes, Fe-NTA showed a much faster increase of and a higher content of &quot;free&quot; iron in the serum than the other two complexes after a single injection of the chelates into rats.(ABSTRACT TRUNCATED AT 250 WORDS)&lt;/p&gt;</p>
キーワード "free" iron ferric nitrilotriacetate (Fe-NTA) ferric ethylenediaminetetraacetate (Fe-EDTA) ferric desferrioxamine (Fe-Desferal Fe-Des) free radecal
Amo Type Article
発行日 1991-12
出版物タイトル Acta Medica Okayama
45巻
6号
出版者 Okayama University Medical School
開始ページ 401
終了ページ 408
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1781297
Web of Science KeyUT A1991GX45300001
JaLCDOI 10.18926/AMO/32110
フルテキストURL fulltext.pdf
著者 Shinji, Toshiyuki| Kyaw, Yi Yi| Gokan, Katsunori| Tanaka, Yasuhito| Ochi, Koji| Kusano, Nobuchika| Mizushima, Takaaki| Fujioka, Shin-ichi| Shiraha, Hidenori| Lwin, Aye Aye| Shiratori, Yasushi| Mizokami, Masashi| Khin, Myo| Miyahara, Masayuki| Okada, Shigeru| Koide, Norio|
抄録 The prevalence of hepatitis C virus (HCV) genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR) amplified. Among them, 35 (31.8%) were of genotype 1, 52 (47.3%) were of genotype 3, and 23 (20.9%) were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.
キーワード hepatitis C virus(HCV)genotype type 6 variant Myanmar Southeast Asia phylogenetic analysis
Amo Type Article
発行日 2004-06
出版物タイトル Acta Medica Okayama
58巻
3号
出版者 Okayama University Medical School
開始ページ 135
終了ページ 142
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 15471435
Web of Sience KeyUT 000222273300004
JaLCDOI 10.18926/AMO/32006
フルテキストURL fulltext.pdf
著者 Wani, Yoji| Notohara, Kenji| Tsukayama, Choutatsu| Okada, Shigeru|
抄録 <p>We performed an immunohistochemical analysis of 2 major DNA mismatch repair proteins, human Mut L homologue-1 (hMLH1) and human Mut S homologue-2 (hMSH2), in hepatocellular carcinoma (HCC) using 33 biopsied and 58 surgically resected specimens, as well as 30 samples from non-cancerous livers. In well-differentiated HCCs, the immunoreactivity for these antigens was well preserved, and the staining intensity was stronger compared to the surrounding liver tissues. However, among 41 moderately-differentiated and 9 poorly-differentiated HCCs of the resected cases, hMLH1- and hMSH2-positive cells were significantly reduced in 19 (38%) and 9 (18%) cases, respectively. In 9 resected tumors, the expression of both of these antigens was reduced. Moreover, in 41 tumors of differing histological grades, 10 and 5 tumors for hMLH1 and hMSH2, respectively, contained a less-differentiated area with a reduced number of immunoreactive cells. The samples from non-cancerous biopsied liver and fetal autopsy tissue were well immunostained for both hMLH1 and hMSH2. We confirmed in this series that the hMLH1 and hMSH2 defect did commonly occur in high-grade HCCs, and that it might play a role in tumor progression.</p>
キーワード hepatocellular carcinoma human Mut L homologue-1(hMLH1) human Mut S homologue-2(hMS2) mismatch repair proteins immunohistochemistry
Amo Type Article
発行日 2001-04
出版物タイトル Acta Medica Okayama
55巻
2号
出版者 Okayama University Medical School
開始ページ 65
終了ページ 71
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11332201
Web of Science KeyUT 000168195700002
JaLCDOI 10.18926/AMO/32004
フルテキストURL fulltext.pdf
著者 Kakehashi, Chikako| Mori, Masaharu| Kawabata, Teruyuki| Okada, Shigeru|
抄録 <p>Administration of ferric nitrilotriacetate (Fe-NTA) in vivo causes acute renal tubular injury and finally induces renal cell carcinoma. There is accumulating evidence that these processes involve free radicals generated by Fe-NTA. To study the mechanism of renal carcinogenesis by Fe-NTA, we attempted to induce malignant transformation of primary cultured renal cells by treatment with Fe-NTA. When primary cultured renal cells (PRC) were treated continuously with Fe-NTA, all of the PRC died without transformation. On the other hand, when PRC were treated intermittently with Fe-NTA, transformed epithelial colonies were observed at 3 weeks after the first treatment. The established transformed cell line (RK523) showed drastic morphological transformation, grew in soft agar, and formed tumors when transplanted into athymic nude mice. These results indicate that the balance between cytotoxicity and mutagenecity is important for Fe-NTA induced transformation. The RK523 cell line may be a useful model for studying renal carcinogenesis in vitro.</p>
キーワード renal cell Fe-NTA(ferric nitrilotriacetate) malignant transformation in vitro
Amo Type Article
発行日 2001-04
出版物タイトル Acta Medica Okayama
55巻
2号
出版者 Okayama University Medical School
開始ページ 97
終了ページ 103
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11332205
Web of Sience KeyUT 000168195700006
JaLCDOI 10.18926/AMO/31968
フルテキストURL fulltext.pdf
著者 Win, Ne| Lwin, Aye Aye| Oo, Myat Mon| Aye, Khin Saw| Soe, Soe| Okada, Shigeru|
抄録 <p>The population of Myanmar comprises 8 major indigenous races (Bamar, Kayin, Kachin, Shan, Rakhine, Mon, Chin, and Kayah). The Bamar reside in the 7 central divisions of the country, and the others reside in the 7 peripheral states that border neighboring countries, including China, Laos, and Thailand in the east and India and Bangladesh in the west. Both malaria and HbE are endemic in Myanmar, although the actual prevalence of the latter in the different indigenous races is not yet known. Hemoglobin electrophoresis was performed in 4 malaria-endemic villages, each having a different predominating indigenous race. The overall prevalence of HbE was 11.4% (52/456 villagers), ranging from 2-6% in the Kayin-predominant villages to 13.1-24.4% in the Bamar-predominant villages. Although the overall HbE prevalence in the villages studied was not significantly different from that of the general Myanmar population, this study strongly documented the influence of racial differences on the prevalence of HbE in Myanmar. To prevent and control severe thalassemia syndromes in Myanmar, extensive prevalence studies of the country?s indigenous races are suggested.</p>
キーワード hemoglobin E malaria indigenous races border areas Myanmar
Amo Type Article
発行日 2005-04
出版物タイトル Acta Medica Okayama
59巻
2号
出版者 Okayama University Medical School
開始ページ 63
終了ページ 66
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16049557
Web of Sience KeyUT 000228590000005
JaLCDOI 10.18926/AMO/31835
フルテキストURL fulltext.pdf
著者 Takayama, Fusako| Nakamoto, Kazuo| Kawasaki, Hiromu| Mankura, Mitsumasa| Egashira, Toru| Ueki, Keiji| Hasegawa, Azusa| Okada, Shigeru| Mori, Akitane|
抄録 <p>Vitis coignetiae Pulliat (Yamabudo) is used as a health juice and wine based on the abundant polyphenols and anthocyanins in its fruit. However, it is not known whether the leaves of this plant confer similar benefits. This study investigated the hepatoprotective effects of aqueous extracts from Vitis coignetiae Pulliat leaves (VCPL) in an animal model of nonalcoholic steatohepatitis (NASH). Rats were fed a choline-deficient high-fat diet for four weeks to generate fatty livers. NASH was induced by oxidative stress loading. Ten weeks later, blood and liver samples were collected from anesthetized animals and assessed biochemically, histologically, and histochemically to determine the extent of oxidative stress injury and the overall effects of VCPL. Six-week VCPL extract supplementation reduced serum levels of liver enzymes, decreased CYP2E1 induction, increased plasma antioxidant activities and delayed the progression of liver fibrosis. The findings suggested that VCPL has strong radical-scavenging activity and may be beneficial in preventing NASH progression.</p>
キーワード Yamabudo nonalcoholic steatohepatitis antioxidant hepatoprotection
Amo Type Original Article
発行日 2009-04
出版物タイトル Acta Medica Okayama
63巻
2号
出版者 Okayama University Medical School
開始ページ 105
終了ページ 111
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 19404342
Web of Science KeyUT 000265457600005