フルテキストURL PlosOne14_1_211505.pdf
著者 Miyoshi, Yuichi| Ohtsuki, Takashi| Kashida, Hiromu| Asanuma, Hiroyuki| Watanabe, Kazunori|
発行日 2019-01-29
出版物タイトル PLoS One
14巻
1号
出版者 PUBLIC LIBRARY SCIENCE
開始ページ e0211505
ISSN 1932-6203
資料タイプ 学術雑誌論文
言語 French
OAI-PMH Set 岡山大学
著作権者 © 2019 Miyoshi et al.
論文のバージョン publisher
PubMed ID 30695081
DOI 10.1371/journal.pone.0211505
Web of Science KeyUT 000457046400041
関連URL isVersionOf https://doi.org/10.1371/journal.pone.0211505
JaLCDOI 10.18926/AMO/56071
フルテキストURL 72_3_257.pdf
著者 Asano, Keiichi| Edamatsu, Midori| F. Hatipoglu, Omer| Inagaki, Junko| Ono, Mitsuaki| Ohtsuki, Takashi| Oohashi, Toshitaka| Hirohata, Satoshi|
抄録 Several research groups demonstrated that ‘a disintegrin-like and metalloproteinase with thrombospondin type 1 motifs (ADAMTS)’-family proteases play roles in cancer progression. However, the origins and contributions of these proteases are not known. Here, we demonstrate an association between host-produced ADAMTS4 and early-stage tumor growth. Murine Lewis lung carcinoma (LLC) tumors showed marked expressions of Adamts4 and Adamts5. We examined the contributions and distributions of host-derived Adamts4 and Adamts5 on tumor growth, using Adamts4LacZ/LacZ and Adamts5LacZ/LacZ knockout mice. Interestingly, the Adamts4LacZ/LacZ mice showed enhanced tumor growth compared to wild-type mice at 5-, 10- and 12-days post-inoculation, whereas the Adamts5LacZ/LacZ mice did not show significant differences in tumor growth. We next examined LacZ distribution in LLC tumor-bearing Adamts4LacZ/LacZ mice by β-galactosidase (β-gal) staining. We found that the β-gal-positive signals were strictly localized at the interior areas of the tumor at 10 days post-inoculation. Multiple staining demonstrated that most of the β-gal-positive cells were localized at the tumor vasculature in Adamts4LacZ/LacZ mice. Interestingly, β-gal-positive signals were not co-localized with biglycan after 10 days post-inoculation, excluding the biglycan cleavage by host-derived ADAMTS4. Taken together, these findings illustrate that host-derived ADAMTS4 was expressed at the tumor vessels and was associated with early-stage tumor growth.
キーワード ADAMTS metalloproteinase extracellular matrix tumor microenvironment mouse
Amo Type Original Article
発行日 2018-06
出版物タイトル Acta Medica Okayama
72巻
3号
出版者 Okayama University Medical School
開始ページ 257
終了ページ 266
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2018 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 29926003
著者 Obika, Masanari| Ogawa, Hiroko| Takahashi, Katsuyuki| Li, Jiayi| Hatipoglu, Omer Faruk| Cilek, Mehmet Zeynel| Miyoshi, Toru| Inagaki, Junko| Ohtsuki, Takashi| Kusachi, Shozo| Ninomiya, Yoshifumi| Hirohata, Satoshi|
発行日 2012-10
出版物タイトル Cancer Science
103巻
10号
資料タイプ 学術雑誌論文