JaLCDOI 10.18926/AMO/32172
フルテキストURL fulltext.pdf
著者 Shimoe, Tosinari| Okada, Yoshio| Tsuji, Takao|
抄録 <p>Wheat germ agglutinin binding to a rat hepatoma cell line dRLa 74 treated with concanavalin A was studied. It increased depending on the concanavalin A concentration in the culture medium. The cells exhibited about twofold increase in wheat germ agglutinin-binding when pretreated with 50 micrograms/ml of concanavalin A for 48 h. The wheat germ agglutinin binding sites were shown to be localized at the cell surface by lectin-histochemistry. Wheat germ agglutinin blotting of microsomal membrane proteins showed a broad wheat germ agglutinin-reactive band with an apparent molecular weight of 90 to 100 kDa. Loss of wheat germ agglutinin binding to dRLa 74 cells and the glycoprotein after neuraminidase treatment suggested that wheat germ agglutinin reacted with cell surface sialyl residues of dRLa 74 cells. The induced change was reversible. Increased wheat germ agglutinin binding returned to the pretreatment level when the concanavalin A-treated cells were subcultured in the absence of concanavalin A. These observations suggest that environmental factors interacting with tumor cell surface sugar moieties may induce reversible epigenetic changes on cell surface carbohydrate structures.</p>
キーワード lectin glycoprotein hepatoma cell line rat
Amo Type Article
発行日 1991-08
出版物タイトル Acta Medica Okayama
45巻
4号
出版者 Okayama University Medical School
開始ページ 275
終了ページ 281
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 1962534
Web of Science KeyUT A1991GD78000010
JaLCDOI 10.18926/AMO/31300
フルテキストURL fulltext.pdf
著者 Yoshida, Atushi| Sotozono, Masaaki| Nakatou, Tatsuaki| Okada, Yoshio| Tsuji, Takao|
抄録 <p>Thomsen-Friedenreich antigen (T antigen) has been supposed to be a cancer-specific carbohydrate antigen. We have previously shown that one third of the Japanese population normally expressed T antigen in gastric surface epithelia and the other two thirds expressed fucosyl-T antigen. Their sialylation and blocked-synthesis were associated with diseased conditions. In the present study, we studied gastric surface epithelial expression of monosaccharide antigen Tn, i.e., a precursor of T antigen, and sialyl-Tn. Normal fundic and pyloric epithelia, respectively, expressed Tn supranucleally and cytoplasmically, but did not express sialyl-Tn. In the intestinal metaplasias and intestinal-type adenomas, goblet cells expressed sialyl-Tn in their vacuoles, and absorptive cells expressed Tn apically. In gastric-type adenomas, Tn, but not sialyl-Tn, was detected. Intestinal-type cancers expressed Tn and sialyl-Tn more often than the diffuse-type cancers. Five cancers did not express Tn, sialyl-Tn, or the T-related antigens. In these, four were diffuse-type cancers. We concluded that: a) normal gastric epithelial cells express Tn; b) metaplastic differentiation is associated with sialylation of Tn and c) expression of Tn and sialyl-Tn is depressed in the gastric cancers. </p>
キーワード Tn immunohistochemistru
Amo Type Article
発行日 1998-08
出版物タイトル Acta Medica Okayama
52巻
4号
出版者 Okayama University Medical School
開始ページ 197
終了ページ 204
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9781270
Web of Sience KeyUT 000075623600004
JaLCDOI 10.18926/AMO/31287
フルテキストURL fulltext.pdf
著者 Okada, Yoshio|
抄録 <p>125I-labeled insulin binding to peripheral human erythrocytes was studied in patients with chronic liver disease. The maximum specific 125I-labeled insulin binding was 12.10 +/- 1.13 %/4 x 10(9) cells (mean +/- SD, n = 10) in normal subjects, and significantly higher in cirrhotic patients (15.32 +/- 1.73 %, n = 11, P less than 0.01) but not in patients with acute and chronic hepatitis (11.44 +/- 2.10 %, n = 3 and 13.2 +/- 1.87 %, n = 7 respectively). The complication of diabetes mellitus significantly increased (P less than 0.05) the maximum insulin binding in chronic hepatitis. Scatchard analysis and average affinity analysis of the binding data suggest that increased insulin binding in cirrhotic patients is due to an increase in the number of insulin binding sites per erythrocytes. The complication of diabetes in chronic liver diseases results in an increase in affinity of insulin binding sites.</p>
キーワード insulin binding erythrocyte liver disease.
Amo Type Article
発行日 1981-06
出版物タイトル Acta Medica Okayama
35巻
3号
出版者 Okayama University Medical School
開始ページ 155
終了ページ 164
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6457506
Web of Science KeyUT A1981MD16600001
JaLCDOI 10.18926/AMO/31272
フルテキストURL fulltext.pdf
著者 Okada, Yoshio|
抄録 <p>Insulin and human erythrocyte cell membrane interactions were studied with respect to binding and dissociation. The per cent of specific binding of 125I-labeled insulin to erythrocytes was directly proportional to the cell concentration. The optimum pH for binding was 8.1. The initial binding rate was directly proportional to, and the steady state insulin binding was reversely proportional to, the incubation temperature. The per cent of specific binding of 125I-labeled insulin was 12.10 +/- 1.13 per cent (mean +/- SD)/4 X 10(9) cells (n = 10) at 0.8 ng/ml insulin. Native insulin competed with 125I-labeled insulin for binding and showed almost complete inhibition at 10(4) ng/ml. The Scatchard plots were upward concave. Maximum binding capacity was 230 binding sites per cell. The average affinity constant decreased as the per cent of fractional occupancy increased. Affinity constants for the empty and filled sites were 1.49 and 0.16 X 10(8) M-1 respectively. Bound insulin was displaced by native insulin. The dissociation rate by &#34;dilution + native insulin&#34; was higher than that by &#34;dilution only&#34;. The dissociation rate was accelerated even by the physiological concentration of insulin and maximum at 100 ng/ml. It is concluded that human erythrocytes have insulin binding sites which are indistinguishable from insulin receptors on the target tissues for insulin.</p>
キーワード insulin binding human erythrocyte.
Amo Type Article
発行日 1981-04
出版物タイトル Acta Medica Okayama
35巻
2号
出版者 Okayama University Medical School
開始ページ 125
終了ページ 135
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6456644
Web of Sience KeyUT A1981LS45700005
JaLCDOI 10.18926/AMO/31255
フルテキストURL fulltext.pdf
著者 Okada, Yoshio| Arima, Terukatsu| Okazaki, Satoru| Nakata, Ken-ichi| Yamabuki, Takahiro| Nagashima, Hideo|
抄録 <p>Insulin binding to erythrocytes was studied in diabetic patients. Insulin binding was lower in untreated diabetics and diabetic patients treated with diet or insulin than in normal subjects. Binding variation was mainly due to decreased binding site concentration in untreated and insulin-treated patients, and to lowered insulin binding site affinity in diet-treated patients. Several patients treated with hypoglycemic agents showed higher insulin binding due to increased binding site concentration. Insulin binding to erythrocytes may not always reflect the insulin binding status of insulin sensitive tissues.</p>
キーワード insulin binding diabetes mellitus erythrocyte.
Amo Type Brief Note
発行日 1981-10
出版物タイトル Acta Medica Okayama
35巻
4号
出版者 Okayama University Medical School
開始ページ 273
終了ページ 277
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6457514
Web of Sience KeyUT A1981MK84200005
JaLCDOI 10.18926/AMO/30781
フルテキストURL fulltext.pdf
著者 Sakai, Nobuyuki| Okada, Yoshio| Tsuji, Takao|
抄録 <p>The serum levels of the carbohydrate antigen sialyl Lewis X (SLEX) increase in liver diseases (Sunayama T, Okada Y, Tsuji T., J Hepatol 1994; 19: 451-458). However, it is not known whether the increased serum SLEX levels are associated with the increased levels of its carrier molecules and/or the increased density of SLEX per carrier molecule. By using of rabbit antibody against an SLEX-positive fraction from HepG2 culture supernatant, we developed an enzyme-linked immunosorbent assay to determine the serum levels of the carrier molecules of SLEX (CM<sub>SLEX</sub>). The CM<sub>SLEX</sub>-levels in patients with hepatocellular carcinoma were significantly higher than those of normal controls (P &#60; 0.001) and benign chronic liver diseases, i.e., chronic active hepatitis, mild and severe form, and liver cirrhosis (P &#60; 0.05). Patients with chronic persistent hepatitis and chronic active hepatitis, mild form, had higher CM<sub>SLEX</sub>-levels than normal controls (P &#60; 0.05). The serum CM<sub>SLEX</sub>-levels did not differ significantly among benign liver diseases. We concluded that serum CM<sub>SLEX</sub>-levels increase nonspecifically in liver diseases. This is a possible molecular mechanism for the increased serum SLEX levels in liver diseases.</p>
キーワード glycoprotein carbohydrate antigen chronic liver disease hepatocellular carcinoma
Amo Type Article
発行日 1997-04
出版物タイトル Acta Medica Okayama
51巻
2号
出版者 Okayama University Medical School
開始ページ 79
終了ページ 85
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9142344
Web of Science KeyUT A1997WX19600004
JaLCDOI 10.18926/AMO/30404
フルテキストURL fulltext.pdf
著者 Yonei, Taiji| Watarai, Shinobu| Okada, Yoshio| Yasuda, Tatsuji| Tsuji, Takao|
抄録 <p>Monoclonal antibodies were raised against urine proteins from diabetic patients. An antibody, YO-2, stained three protein bands with apparent molecular weights of 66, 49, and 36 kDa. These bands were not reactive with an anti-human albumin antibody. The urine levels of YO-2-reactive antigen in the normal control were 0.97 +/- 0.37 U/g-Cr (units per gram of urine creatinine) (mean +/- SD). Those of the normo-, micro-, and macroalbuminuric diabetic patients, respectively, were 1.38 +/- 1.36, 2.87 +/- 2.07, and 3.92 +/- 3.33 U/g-Cr. They were significantly higher in the micro- and macroalbuminuric patients. The urine levels of YO-2-reactive antigen had no significant correlation with the urine albumin levels and hemoglobin A1c. We concluded that; a) monoclonal antibody YO-2 recognized a non-albumin urine antigen increasingly excreted in diabetic patients with nephropathy, b) recent glycemic control of diabetes would not significantly affect the urinary excretion rate of YO-2-reactive antigen, and c) the excretion rate and probably the mechanism of YO-2-reactive protein differed from those of albumin. The urine levels of YO-2-reactive antigen could be a clinical marker of diabetic nephropathy.</p>
キーワード diabetes nephropathy monoclonal antibody microalbuminuria
Amo Type Article
発行日 1995-06
出版物タイトル Acta Medica Okayama
49巻
3号
出版者 Okayama University Medical School
開始ページ 153
終了ページ 159
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7676846
Web of Sience KeyUT A1995RH05400006