このエントリーをはてなブックマークに追加
ID 32973
フルテキストURL
Thumnail fulltext.pdf 2.33 MB
著者
Fleischmajer, Raul Mount Sinai School of Medicine, New York
Utani, Atsushi National Institute of Dental Research, National Institutes of Health
Douglas MacDonald II, E. Mount Sinai School of Medicine, New York
Perlish, Jerome S Mount Sinai School of Medicine, New York
Pan, Te-Cheng Thomas Jefferson University
Chu, Mon-Li Thomas Jefferson University
Nomizu, Motoyoshi National Institute of Dental Research, National Institutes of Health
Ninomiya, Yoshifumi Okayama University
Yamada, Yoshihiko National Institute of Dental Research, National Institutes of Health
抄録

To study the mechanism of basement membrane formation, we determined by immunochemistry temporal and spatial expression of laminin-5 (Ln-5), laminin-1 (Ln-1) and their integrin receptors during early skin morphogenesis. A 3-dimensional skin culture was used that allows the study of the sequential molecular events of basement membrane formation at the epidermodermal interface. During early anchorage of keratinocytes to the extracellular matrix there is expression of Ln-5, BP-230 antigen and α3, β1 integrin subunits. During epidermal stratification and prior to the formation of the lamina densa there is assembly of Ln-5, Ln-1, collagen IV and nidogen accompanied by keratinocyte basal clustering of α2, α3, α6, β1, and β4 integrin subunits. The assembly pattern of Ln-1 and Ln-5 can be disturbed with functional antibodies against the β1 (AIIB2) and α6 (GoH3) integrin subunits. Ln-1 assembly can also be disturbed with antibodies against its E8 domain and by competitive inhibition with a synthetic peptide (AG-73) derived from its G-4 domain. Quantitative RT-PCR showed that the dermis contributes about 80% of the laminin γ1 chain mRNA while 20% is produced by the epidermis which emphasizes its dual tissue origin and the major contribution of the mesenchyma in laminin production. The laminin γ2 chain mRNA, present in Ln-5, was mostly of epidermal origin. This study presents evidence that during the initiation of basement membrane formation, laminins bind to keratinocyte plasma membrane receptors and thus may serve as nucleation sites for further polymerization of these compounds by a self-assembly process.

キーワード
Basement membrane
Laminin
Integrin
備考
Published with permission from the copyright holder. This is the institute's copy, as published in Journal of Cell Science, 30 July 1998, Volume 111, Issue 14, 1998, Pages 1929-1940.
Publisher URL:http://jcs.biologists.org/cgi/reprint/111/14/1929
Direct access to Thomson Web of Science record
Copyright 1998 © The Company of Biologists Ltd. All rights reserved.
発行日
1998-7
出版物タイトル
Journal of Cell Science
111巻
14号
開始ページ
1929
終了ページ
1940
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
査読
有り
Web of Sience KeyUT
Submission Path
biology_general/7