Wajima, Takeaki Graduate School of Comprehensive Human Sciences, University of Tsukuba
Sabui, Subrata National Institute of Cholera and Enteric Diseases
Fukumoto, Megumi Graduate School of Comprehensive Human Sciences, University of Tsukuba
Kano, Shigeyuki Graduate School of Comprehensive Human Sciences, University of Tsukuba
Ramamurthy, Thandavarayan National Institute of Cholera and Enteric Diseases
Chatterjee, Nabendu Sekhar National Institute of Cholera and Enteric Diseases
Hamabata, Takashi Graduate School of Comprehensive Human Sciences, University of Tsukuba
Enterotoxigenic Escherichia coli (ETEC) produces a variety of colonization factors necessary for attachment to the host cell, among which CS6 is one of the most prevalent in ETEC isolates from developing countries. The CS6 operon is composed of 4 genes, cssA, cssB, cssC, and cssD. The molecular mechanism of CS6 assembly and cell surface presentation, and the contribution of each protein to the attachment of the bacterium to intestinal cells remain unclear. In the present study, a series of css gene-deletion mutants of the CS6 operon were constructed in the ETEC genetic background, and their effect on adhesion to host cells and CS6 assembly was studied. Each subunit deletion resulted in a reduction in the adhesion to intestinal cells to the same level of laboratory E. coli strains, and this effect was restored by complementary plasmids, suggesting that the 4 proteins are necessary for CS6 expression. Bacterial cell fractionation and western blotting of the mutant strains suggested that the formation of a CssA–CssB–CssC complex is necessary for recognition by CssD and transport of CssA–CssB to the outer membrane as a colonization factor.
Enterotoxigenic Escherichia coli
©2011 Elsevier Ltd. All rights reserved.
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