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ID 31536
JaLCDOI
フルテキストURL
著者
Hashimoto, Kozo Okayama University
Hattori, Teruhiko Okayama University
Murakami, Kazuharu Okayama University
Suemaru, Shouso Okayama University
Kawada, Yoshiro Okayama University
Ota, Zensuke Okayama University
抄録

The stability of rat (human) CRF in serum, urine and tissue incubation medium was examined using Sephadex gel filtration and CRF radioimmunoassay with anti-rat (human) CRF serum. Human serum after incubation with rat (human) CRF for 1 h at 37 degrees C showed two peaks of CRF immunoreactivity on a Sephadex G-50 fine column. Most of the immunoreactivity coeluted with the rat (human) CRF marker. When rat (human) CRF was incubated with rat liver, kidney or hypothalamus, only 3.1-14.9% of the CRF was recovered at the rat (human) CRF position on gel filtration, and two to four CRF-immunoreactive peaks appeared after the rat (human) CRF marker. When rat (human) CRF was incubated with human urine (pH 6.0) for 24 h at room temperature, one peak of CRF immunoreactivity coeluted with the rat (human) CRF marker on Sephadex gel filtration. The urine extracts of normal rats showed some small peaks of CRF-like immunoreactivity on the Sephadex column, with the main peak appearing after authentic CRF. These results suggest that rat (human) CRF is relatively stable in serum and urine, but is easily degraded by tissue enzymes, with the degraded CRF fragments being excreted in the urine.

キーワード
rat(human) corticotropin releasing factor
sephadex chromatography
stability
Amo Type
Article
発行日
1985-06
出版物タイトル
Acta Medica Okayama
39巻
3号
出版者
Okayama University Medical School
開始ページ
199
終了ページ
206
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT