このエントリーをはてなブックマークに追加
ID 34112
フルテキストURL
著者
Taguchi, Fumiko The Graduate School of Natural Science and Technology, Okayama University
Ogawa, Yujiro The Graduate School of Natural Science and Technology, Okayama University
Takeuchi, Kasumi The Graduate School of Natural Science and Technology, Okayama University
Suzuki, Tomoko The Graduate School of Natural Science and Technology, Okayama University
Toyoda, Kazuhiro The Graduate School of Natural Science and Technology, Okayama University
Shiraishi, Tomonori The Graduate School of Natural Science and Technology, Okayama University
Ichinose, Yuki The Graduate School of Natural Science and Technology, Okayama University
抄録
Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modification of flagellin. Although the function of Orf3 remained unclear, an orf3 deletion mutant (Delta orf3 mutant) had reduced virulence on tobacco plants. Orf3 shows significant homology to a 3-oxoacyl-(acyl carrier protein) synthase III in the fatty acid elongation cycle. The Delta orf3 mutant had a significantly reduced ability to form acyl homoserine lactones (AHLs), which are quorum-sensing molecules, suggesting that Orf3 is required for AHL synthesis. In comparison with the wild-type strain, swarming motility, biosurfactant production, and tolerance to H2O2 and antibiotics were enhanced in the Delta orf3 mutant. A scanning electron micrograph of inoculated bacteria on the tobacco leaf surface revealed that there is little extracellular polymeric substance matrix surrounding the cells in the Delta orf3 mutant. The phenotypes of the Delta orf3 mutant and an AHL synthesis (Delta psyI) mutant were similar, although the mutant-specific characteristics were more extreme in the Delta orf3 mutant. The swarming motility of the Delta orf3 mutant was greater than that of the Delta psyI mutant. This was attributed to the synergistic effects of the overproduction of biosurfactants and/or alternative fatty acid metabolism in the Delta orf3 mutant. Furthermore, the amounts of iron and biosurfactant seem to be involved in biofilm development under quorum-sensing regulation in P. syringae pv. tabaci 6605.
キーワード
TO-CELL SIGNALS
AERUGINOSA
FLAGELLIN
BIOFILMS
MOTILITY
IRON
IDENTIFICATION
SIDEROPHORES
SPECIFICITY
FLUORESCENT
備考
Digital Object Identifer:10.1128/JB.00763-06
Published with permission from the copyright holder. This is the author's copy, as published in the Journal of Bacteriology, Dec 2006, Volume 188, Issue 24, Pages 8376-8384.
Publisher URL:http://dx.doi.org/10.1128/JB.00763-06
Direct access to Thomson Web of Science record
Copyright © 2006 American Society for Microbiology
発行日
2006-12
出版物タイトル
Journal of Bacteriology
188巻
24号
出版者
American Society for Microbiology
開始ページ
8376
終了ページ
8384
ISSN
0021-9193
NCID
AA0069403X
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
American Society for Microbiology
論文のバージョン
author
査読
有り
DOI
PubMed ID
Web of Sience KeyUT
Submission Path
microbiology_and_immunology/7