フルテキストURL Plant_Cell_Physiol_58_8_1583.pdf Fig.pdf suppl_fig.pdf
著者 Cai, Qingqing| Fukushima, Hiroko| Yamamoto, Mai| Ishii, Nami| Sakamoto, Tomoaki| Kurata, Tetsuya| Motose, Hiroyasu| Takahashi, Taku|
抄録 The acaulis5 (acl5) mutant of Arabidopsis thaliana is defective in the biosynthesis of thermospermine and shows a dwarf phenotype associated with excess xylem differentiation. SAC51 was identified from a dominant suppressor of acl5, sac51-d, and encodes a basic helix-loop-helix protein. The sac51-d mutant has a premature termination codon in an upstream open reading frame (uORF) that is conserved among all four members of the SAC51 family, SAC51 and SACL1-SACL3 This suggests that thermospermine cancels the inhibitory effect of the uORF in main ORF translation. Another suppressor, sac57-d, has a mutation in the conserved uORF of SACL3 To define further the function of the SAC51 family in the thermospermine response, we analyzed T-DNA insertion mutants of each gene. Although sacl1-1 may not be a null allele, the quadruple mutant showed a semi-dwarf phenotype but with an increased level of thermospermine and decreased sensitivity to exogenous thermospermine that normally represses xylem differentiation. The sac51-1 sacl3-1 double mutant was also insensitive to thermospermine. These results suggest that SAC51 and SACL3 play a key role in thermospermine-dependent negative control of thermospermine biosynthesis and xylem differentiation. Using 5' leader-GUS (β-glucuronidase) fusion constructs, however, we detected a significant enhancement of the GUS activity by thermospermine only in SAC51 and SACL1 constructs. Furthermore, while acl5-1 sac51-1 showed the acl5 dwarf phenotype, acl5-1 sacl3-1 exhibited an extremely tiny-plant phenotype. These results suggest a complex regulatory network for the thermospermine response in which SAC51 and SACL3 function in parallel pathways.
キーワード Arabidopsis Thermospermine Translation Xylem differentiation uORF
備考 This is an Accepted Manuscript of an article published by Oxford University Press
発行日 2016-08
出版物タイトル Plant and Cell Physiology
57巻
8号
出版者 Japanese Society of Plant Physiologists
開始ページ 1583
終了ページ 1592
ISSN 0032-0781
NCID AA0077511X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 http://creativecommons.org/licenses/by-nc-nd/4.0/
論文のバージョン author
PubMed ID 27388339
DOI 10.1093/pcp/pcw113
Web of Sience KeyUT 000384717100001
関連URL https://doi.org/10.1093/pcp/pcw113
著者 Yoshimoto, Kaori| Takamura, Hiroyoshi| Kadota, Isao| 本瀬 宏康| Takahashi, Taku|
発行日 2016-02-16
出版物タイトル Scientific Reports
6巻
資料タイプ 学術雑誌論文
著者 Takatani, Shogo| Otani, Kento| Kanazawa, Mai| 髙橋 卓| 本瀬 宏康|
発行日 2015-11
出版物タイトル Journal of Plant Research
128巻
6号
資料タイプ 学術雑誌論文
フルテキストURL Takatani ABA SR15 srep11364.pdf
著者 Takatani, Shogo| Hirayama, Takashi| Hashimoto, Takashi| Takahashi, Taku| Motose, Hiroyasu|
抄録 Abscisic acid (ABA) regulates seed maturation, germination and various stress responses in plants. The roles of ABA in cellular growth and morphogenesis, however, remain to be explored. Here, we report that ABA induces the ectopic outgrowth of epidermal cells in Arabidopsis thaliana. Seedlings of A. thaliana germinated and grown in the presence of ABA developed ectopic protrusions in the epidermal cells of hypocotyls, petioles and cotyledons. One protrusion was formed in the middle of each epidermal cell. In the hypocotyl epidermis, two types of cell files are arranged alternately into non-stoma cell files and stoma cell files, ectopic protrusions being restricted to the non-stoma cell files. This suggests the presence of a difference in the degree of sensitivity to ABA or in the capacity of cells to form protrusions between the two cell files. The ectopic outgrowth was suppressed in ABA insensitive mutants, whereas it was enhanced in ABA hypersensitive mutants. Interestingly, ABA-induced ectopic outgrowth was also suppressed in mutants in which microtubule organization was compromised. Furthermore, cortical microtubules were disorganized and depolymerized by the ABA treatment. These results suggest that ABA signaling induces ectopic outgrowth in epidermal cells through microtubule reorganization.
キーワード Cell growth Plant cytoskeleton
備考 Scientific Reports 5, Article number: 11364 (2015), © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. This work is licensed under a Creative Commons Attribution 4.0 International License.
発行日 2015-06-12
出版物タイトル Scientific Reports
5巻
出版者 Nature Publishing Group
開始ページ 11364
ISSN 2045-2322
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © 2015 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
論文のバージョン publisher
PubMed ID 26068445
DOI 10.1038/srep11364
Web of Sience KeyUT 000356152700001
著者 Motose, Hiroyasu| Takatani, Shogo| Ikeda, Tatsuya| Takahashi, Taku|
発行日 2012-12
出版物タイトル Plant Signaling & Behavior
7巻
12号
資料タイプ 学術雑誌論文
著者 Inoue, Genki| Kaneta, Takashi| Takayanagi, Toshio| Kakehi, Junichi| Motose, Hiroyasu| Takahashi, Taku|
発行日 2013-05
出版物タイトル Analytical Methods
5巻
11号
資料タイプ 学術雑誌論文
著者 Yoshimoto, Kaori| Noutoshi, Yoshiteru| Hayashi, Ken-ichiro| Shirasu, Ken| Takahashi, Taku| Motose, Hiroyasu|
発行日 2012-04
出版物タイトル Plant and Cell Physiology
53巻
4号
資料タイプ 学術雑誌論文
著者 Yoshimoto, Kaori| Noutoshi, Yoshiteru| Hayashi, Ken-ichiro| Shirasu, Ken| Takahashi, Taku| Motose, Hiroyasu|
発行日 2012-08
出版物タイトル Plant Signaling & Behavior
7巻
8号
資料タイプ 学術雑誌論文
著者 Motose, Hiroyasu| Hamada, Takahiro| Yoshimoto, Kaori| Murata, Takashi| Hasebe, Mitsuyasu| Watanabe, Yuichiro| Hashimoto, Takashi| Sakai, Tatsuya| Takahashi, Taku|
発行日 2011-09
出版物タイトル The Plant Journal
67巻
6号
資料タイプ 学術雑誌論文
著者 Imai, Akihiro| Hanzawa, Yoshie| Komura, Mio| Yamamoto, Kotaro T.| Komeda, Yoshibumi| Takahashi, Taku|
発行日 2006-09-15
出版物タイトル Development
133巻
18号
資料タイプ 学術雑誌論文