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ID 55316
フルテキストURL
著者
Takatani, Shogo Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University
Ozawa, Shinichiro Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University
Yagi, Noriyoshi Graduate School of Biological Science, Nara Institute of Science and Technology
Hotta, Takashi Graduate School of Biological Science, Nara Institute of Science and Technology
Hashimoto, Takashi Graduate School of Biological Science, Nara Institute of Science and Technology
Takahashi, Yuichiro Graduate School of Natural Science and Technology/Faculty of Science, Okayama University Kaken ID researchmap
Takahashi, Taku Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID researchmap
Motose, Hiroyasu Department of Biological Science, Graduate School of Natural Science and Technology, Okayama University ORCID Kaken ID publons researchmap
抄録
 Plant cortical microtubules align perpendicular to the growth axis to determine the direction of cell growth. However, it remains unclear how plant cells form well-organized cortical microtubule arrays in the absence of a centrosome. In this study, we investigated the functions of Arabidopsis NIMA-related kinase 6 (NEK6), which regulates microtubule organization during anisotropic cell expansion. Quantitative analysis of hypocotyl cell growth in the nek6-1 mutant demonstrated that NEK6 suppresses ectopic outgrowth and promotes cell elongation in different regions of the hypocotyl. Loss of NEK6 function led to excessive microtubule waving and distortion, implying that NEK6 suppresses the aberrant cortical microtubules. Live cell imaging showed that NEK6 localizes to the microtubule lattice and to the shrinking plus and minus ends of microtubules. In agreement with this observation, the induced overexpression of NEK6 reduced and disorganized cortical microtubules and suppressed cell elongation. Furthermore, we identified five phosphorylation sites in β-tubulin that serve as substrates for NEK6 in vitro. Alanine substitution of the phosphorylation site Thr166 promoted incorporation of mutant β-tubulin into microtubules. Taken together, these results suggest that NEK6 promotes directional cell growth through phosphorylation of β-tubulin and the resulting destabilization of cortical microtubules.
キーワード
Cell growth
Microtubules
Plant cytoskeleton
備考
Published in Scientific Reports. 7, 7826(2017)
発行日
2017-08-10
出版物タイトル
Scientific Reports
7巻
出版者
Nature Publishing Group
開始ページ
7826
ISSN
20452322
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1038/s41598-017-08453-5