Okayama University Medical School Acta Medica Okayama 19 1 1965 Immunochemical analysis of antigenic properties of human stomach cancer EN Chiaki Tai Article <p>From the data presented in this communication, it might be concluded that a cancer specific substance, which can be demonstrated in gel diffusion, is present in human cancer tissue, common to various epithelial cancers of different individuals, although it may vary in its concentration. Needless to say, this substance is quite different from the so-called interspecies antigen or organ specific antigen, as proved by the present experiments. Furthermore, this substance can be eluted well by the Fluorocarbon treatment and it displays physically and chemically unstable characteristics. This substance is likely to be included in the microsome fraction and soluble fraction which was determined by gel diffusion technique. However, the association of this substance with other specific antigenic substances of human cancer, concerned with &#34;delayed type skin reaction&#34;, &#34;cytopathogenic antiserum against cancer cell&#34;, and &#34;complement fixing antibody in serum of patients with cancer&#34;, has not been elucidated in this study.</p> No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 19 1 1965 Immunochemical analysis of antigenic properties of MH134 ascitic hepatoma EN Chiaki Tai Article <p>According to the data obtained in this experiment by means of the geldiffusion technique, the specific antigen was not detected in MH134 ascitic tumor, comparing the anti-C3H liver sera with anti-MH 134 tumor sera, though a loss of organ specific antigen and weak antigenicity were found in MH134 tumor extract. In order to detect some qualitative alteration, supposedly a gain in antigenic components, the transplant rejection test was carried out. The result of this test indicates that the relative not absolute resistance could be induced to C3H mice by this prior sensitization with cell free active extract eluted from MH134 tumor tissue by Fluorocarbon treatment. During these experiments, it became clear that MH134 ascitic tumor cell has weak immunizing properties so that prolonged lapse of time and large dose of antigen are inevitably necessary. Moreover, through Fluorocarbon treatment of the tumor homogenate, the cellfree, serologically active antigen could be obtained, which will serve well for the induction of the isologous immunization.</p> No potential conflict of interest relevant to this article was reported.