著者 Sawada, Takahiro| Eguchi, Miho| Asaki, Seiya| Kashiwagi, Ryota| Shimomura, Kousuke| Taguchi, Fumiko| Matsui, Hidenori| Yamamoto, Mikihiro| Noutoshi, Yoshiteru| Toyoda, Kazuhiro| Ichinose, Yuki|
抄録 Our previous studies revealed that flagellar-motility-defective mutants such as ∆fliC of Pseudomonas syringae pv. tabaci 6605 (Pta6605) have remarkably reduced production of N-acyl-homoserine lactones (AHL), quorum-sensing molecules. To investigate the reason of loss of AHL production in ∆fliC mutant, we carried out transposon mutagenesis. Among approximately 14,000 transconjugants, we found 11 AHL production-recovered (APR) strains. In these APR strains, a transposon was inserted into either mexE or mexF, genes encoding for the multidrug efflux pump transporter MexEF-OprN, and mexT, a gene encoding a putative transcriptional activator for mexEF-oprN. These results suggest that MexEF-OprN is a negative regulator of AHL production. To confirm the negative effect of MexEF-OprN on AHL production, loss- and gain-of-function experiments for mexEF-oprN were carried out. The ∆fliC∆mexF and ∆fliC∆mexT double mutant strains recovered AHL production, whereas the mexT overexpressing strain abolished AHL production, although the psyI, a gene encoding AHL synthase, is transcribed as wild type. Introduction of a mexF or mexT mutation into another flagellar-motility- and AHL production-defective mutant strain, ∆motCD, also recovered the ability to produce AHL. Furthermore, introduction of the mexF mutation into other AHL production-defective mutant strains such as ∆gacA and ∆aefR also recovered AHL production but not to the ∆psyI mutant. These results indicate that MexEF-OprN is a decisive negative determinant of AHL production and accumulation.
キーワード Flagella motility MexEF-OprN Multidrug efflux pump transporter N-Acyl-homoserine lactone Quorum sensing
備考 This is an Accepted Manuscript of an article published by Springer Nature 2018 This fulltext will be available in Mar 2019
発行日 2018-03-16
出版物タイトル Molecular Genetics and Genomics
出版者 Springer
開始ページ 1
終了ページ 11
ISSN 1617-4615
NCID AA11547886
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 29549432
DOI 10.1007/s00438-018-1430-9
関連URL isVersionOf https://doi.org/10.1007/s00438-018-1430-9
著者 Marutani, Mizuri| Taguchi, Fumiko| Ogawa, Yujiro| Hossain, Mijan Md.| Yoshishige, Inagaki| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
発行日 2008-04
出版物タイトル Molecular Genetics and Genomics
279巻
4号
資料タイプ 学術雑誌論文
著者 Taguchi, Fumiko| Ogawa, Yujiro| Takeuchi, Kasumi| Suzuki, Tomoko| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
発行日 2006-12
出版物タイトル Journal of Bacteriology
188巻
24号
資料タイプ 学術雑誌論文
著者 Takeuchi, Kasumi| Ono, Hiroshi| Yoshida, Mitsuru| Ishii, Tadashi| Katoh, Etsuko| Taguchi, Fumiko| Miki, Ryuji| Murata, Katsuyoshi| Kaku, Hanae| Ichinose, Yuki|
発行日 2007-10
出版物タイトル Journal of Bacteriology
189巻
19号
資料タイプ 学術雑誌論文
著者 Taguchi, Fumiko| Shibata, Satoshi| Suzuki, Tomoko| Ogawa, Yujiro| Aizawa, Shin-Ichi| Takeuchi, Kasumi| Ichinose, Yuki|
発行日 2008-01-01
出版物タイトル Journal of Bacteriology
190巻
2号
資料タイプ 学術雑誌論文
著者 Taguchi, Fumiko| Takeuchi, Kasumi| Katoh, Etsuko| Murata, Katsuyoshi| Suzuki, Tomoko| Marutani, Mizuri| Kawasaki, Takayuki| Eguchi, Minako| Katoh, Shizue| kaku, Hanae| Yasuda, Chihiro| Inagaki, Yoshishige| Toyoda, Kazuhiro| Shiraishi, Tomonori| Ichinose, Yuki|
発行日 2006-6
出版物タイトル Cellular Microbiology
8巻
6号
資料タイプ 学術雑誌論文