著者 Takahashi, Fumio| Hatase, Osamu| Kuramitsu, Makoto| Itano, Toshifumi| Nisida, Isamu|
発行日 1977-02
出版物タイトル Acta Medica Okayama
31巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32071
フルテキストURL fulltext.pdf
著者 Takahashi, Fumio| Kuramitsu, Makoto| Tokuda, Masaaki| Matsui, Hideki| Itano, Toshifumi| Murakami, Tetsu-Hide| Hatase, Osamu| Nishida, Isamu|
抄録 <p>Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.</p>
キーワード chick growth factors cell proliferation growth regulation DNA and RNA synthesis protein synthesis
Amo Type Article
発行日 1979-06
出版物タイトル Acta Medica Okayama
33巻
3号
出版者 Okayama University Medical School
開始ページ 167
終了ページ 176
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 158945
JaLCDOI 10.18926/AMO/32056
フルテキストURL fulltext.pdf
著者 Hatase, Osamu| Fujii, Toshitake| Kuramitsu, Makoto| Itano, Toshifumi| Takahashi, Fumio| Murakami, Tetsuhide| Nishida, Isamu|
抄録 Factors that inhibit and stimulate cell proliferation were found to coexist in rat liver supernatant. The inhibitory and stimulatory factors were separated by ethanol fractionation. Both factors were sensitive to heat- and trypsin-treatment. The activity of the inhibitor was diminished by partial hepatectomy. The inhibitor from normal livers inhibited DNA and RNA synthesis in the L-cell system, but the same fraction from regenerating livers caused little or no inhibition of nucleic acid synthesis. The stimulatory factor from regenerating livers had a stronger effect on cell proliferation than that of normal livers. Furthermore, the inhibitor from normal livers depressed DNA synthesis in vivo in regenerating livers.
キーワード growth factors growth stimulants growth inhibitants rat liver cytoplasm cell proliferation
Amo Type Article
発行日 1979-04
出版物タイトル Acta Medica Okayama
33巻
2号
出版者 Okayama University Medical School
開始ページ 73
終了ページ 80
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 157671