JaLCDOI 10.18926/AMO/31641
フルテキストURL fulltext.pdf
著者 Kawahara, Nobuaki| Ohta, Masahiro| Liu, Miao| Taga, Hiroko| Taketa, Kazuhisa| Kudo, Takafumi|
抄録 <p>Our purpose was to investigate developmental alterations of human alpha-fetoprotein (AFP) oligosaccharides in maternal serum by lectin affinity electrophoresis and to compare the AFP glycoforms in maternal serum with those in umbilical cord serum and amniotic fluid. AFP glycoforms were separated by affinity electrophoresis with concanavalin A (Con A), lentil lectin (LCA), erythroagglutinating phytohemagglutinin (E-PHA) and Allomyrina dichotoma lectin (allo A) and detected by sensitive antibody affinity blotting. In maternal serum, increased proportions of Con A-nonreactive AFP (AFP-C1), LCA strongly-reactive AFP (AFP-L3) and E-PHA-reactive AFP (AFP-P4 and AFP-P5) decreased gradually during the early gestational weeks. Allo A-nonreactive AFP (AFP-A1 and asialo-AFP) were found only in amniotic fluids during early gestational weeks. The percentages of these glycoforms at full term were almost the same among those body fluids. Since the glycoforms of maternal serum AFP were close to those of umbilical cord serum AFP, lectin-affinity electrophoretic analysis of maternal serum AFP may be useful for evaluating the developmental state of fetus by examining the nature of AFP sugar chain.</p>
キーワード alpha-fetoprotein affinity electrophoresis lectin maternal alpha-fetoprotein
Amo Type Article
発行日 1999-06
出版物タイトル Acta Medica Okayama
53巻
3号
出版者 Okayama University Medical School
開始ページ 103
終了ページ 110
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
Web of Sience KeyUT 000081201100001
JaLCDOI 10.18926/AMO/31342
フルテキストURL fulltext.pdf
著者 Ohta, Masahiro| Kawahara, Nobuaki| Liu, Miao| Taketa, Kazuhisa| Kudo, Takafumi| Taga, Hiroko|
抄録 <p>Affinity electrophoresis of human alpha-fetoprotein (AFP) in amniotic fluid from pregnant women between 6 to 42 weeks of gestation and in the serum of a yolk sac tumor was performed with concanavalin A (Con A), lentil lectin (LCA), erythroagglutinating phytohemagglutinin (E-PHA) and Allomyrina dichotoma lectin (allo A). Separated AFP bands were detected by sensitive antibody-affinity blotting. In the first trimester, amniotic fluid AFP showed elevated percentages of Con A-nonreacting AFP (AFP-C1) and LCA weakly-reacting AFP (AFP-L2) as previously reported. Additionally, high percentages of E-PHA strongly-reacting AFP (AFP-P5) and E-PHA-reacting AFP (AFP-P4) were observed. E-PHA-nonreacting AFP (AFP-P1), E-PHA weakly-reacting AFP (AFP-P3f), allo A-nonreacting AFP (AFP-A1) and asialo-AFP, AFP-A1 s, were present only in amniotic fluids from 6 to 17 weeks of gestation. With advancing gestation, percentages of AFP-C1, AFP-P4 and AFP-P5 decreased and AFP-L2, AFP-P3f, AFP-A1, and AFP-A1 s disappeared by the end of 18 weeks. The glycoforms of serum AFP of the yolk sac tumor resembled those of amniotic fluid AFP in the early gestational stages. </p>
キーワード ?-fetoprotein affinity electrophoresis lectin amniotic fluid yolk sac tumor
Amo Type Article
発行日 1998-02
出版物タイトル Acta Medica Okayama
52巻
1号
出版者 Okayama University Medical School
開始ページ 27
終了ページ 33
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9548991
Web of Sience KeyUT 000072264100004
JaLCDOI 10.18926/AMO/30507
フルテキストURL fulltext.pdf
著者 Ogata, Masatoshi| Taga, Hiroko| Taketa, Kazuhisa|
抄録 <p>&#60;P&#62;Microdetermination of alpha-fetoprotein (AFP) glycoforms by lectin affinity electrophoresis followed by chemiluminescence reaction using horseradish peroxidase (POD) or alkaline phosphatase (ALP) in antibody-affinity blotting was developed. The intensity of chemiluminescence obtained by ALP was greater than that by POD; however, the coefficient of variation with POD was less than that with ALP. The optimized sensitivity of the chemiluminescence method with POD was two times that of the most sensitive colorimetric method currently available in terms of the chemiluminescence intensity per unit AFP concentration. The lower detection limit by the chemiluminescence method with POD (0.5 ng/ml) was much lower than that by the colorimetric method (3 ng/ml). Both methods gave identical percentages of lentil lectin- and erythroagglutinating phytohemagglutinin-reactive minor bands using a serum with 52 ng/ml AFP. This result indicates that microdetermination of AFP glycoforms by chemiluminescence after lectin-affinity electrophoresis was more sensitive than currently available methods and that it is potentially useful for clinical application&#60;/P&#62;</p>
キーワード alpha-fetoprotein lectin affinity electrophoresis chemiluminescence antibode-affinity biotting avidin-biotin complex
Amo Type Article
発行日 1996-06
出版物タイトル Acta Medica Okayama
50巻
3号
出版者 Okayama University Medical School
開始ページ 119
終了ページ 124
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8805850
Web of Sience KeyUT A1996UU60400001
JaLCDOI 10.18926/AMO/30308
フルテキストURL fulltext.pdf
著者 Taketa, Kazuhisa| Ichikawa, Eriko| Taga, Hiroko| Hirai, Hidematsu|
抄録 <p>&lt;p&gt;A combination of agarose gel electrophoresis and a newly developed technique of electro-affinity transfer was applied to the detection of circulating immune complexes of human alpha-fetoprotein (AFP) and anti-AFP. After electrophoretic transfer to nitrocellulose membrane, to which affinity-purified polyclonal horse antibodies to human AFP were bound, the membranes were treated with or without rabbit immunoglobulins to human AFP, followed by overlaying with horseradish peroxidase-labeled goat anti-rabbit IgG for color development. Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. The complexes were stained 20-40% as dark as the equivalent amount of free AFP by treatment with rabbit anti-AFP, and 10-20% as dark without the antibody treatment over a wide range of antigen-antibody ratios.&lt;/p&gt;</p>
キーワード circulating immune complexes ?-fetoprotein electrophoresis electroaffinity transfer enzyme immuno-detection
Amo Type Brief Note
発行日 1984-08
出版物タイトル Acta Medica Okayama
38巻
4号
出版者 Okayama University Medical School
開始ページ 409
終了ページ 413
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6208755
Web of Sience KeyUT A1984TG25900011