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ID 30788
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 4.02 MB
著者
Matsuo, Toshihiko Okayama University
Takabatake, Mayumi Okayama University
Matsuo, Nobuhiko Okayama University
抄録

Retinal cells from chick embryos aged 7.5 days of gestation were cultured for two months in a non-adherent suspension culture dish to study the effects of growth factors and co-culture with retinal pigment epithelial cells on their differentiation. Dissociated retinal cells became cellular aggregates (multicellular spheroids) within a day, and rosettes were formed in the spheroids after 2 days. Ultrastructurally, neurons of the rosettes developed connecting cilia, ellipsoids (accumulation of mitochondria), and external limiting membrane, indicative of their differentiation into photoreceptor cells. Epidermal growth factor enhanced the expression of rhodopsin by rosette-forming neurons, while basic fibroblast growth factor induced the growth of Mueller cells at 4 weeks, and their transdifferentiation into lens-epithelial-like cells at 8 weeks. Co-culture of retinal cells with retinal pigment epithelial cells enhanced the formation of rosettes in spheroids. Multicellular spheroids formed in a dish for suspension culture would provide a convenient in vitro system to examine differentiation and transdifferentiation of the retina.

キーワード
multicellular spheroids
differentiation
transdifferentiation
retina
retinal pigment epithelium
growth factors
Amo Type
Article
発行日
1997-10
出版物タイトル
Acta Medica Okayama
51巻
5号
出版者
Okayama University Medical School
開始ページ
251
終了ページ
260
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT