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ID 31563
JaLCDOI
フルテキストURL
著者
Nouso, Kazuhiro Okayama University
Battula, Narayama Food &Drug Administration
Thorgeirsson, Snorri S Natonal Cancer Institute NIH
Higashi, Toshihiro Okayama University
Tsuji, Takao Okayama University
抄録

We expressed mouse cytochrome P1-450 and P3-450 using recombinant vaccinia virus gene expression system in HeLa cells that were devoid of significant basal levels of P-450. HeLa cells were infected with the recombinant vaccinia virus containing either mouse cytochrome P1-450 or P3-450 cDNA, and the cell lysates were analyzed for the kinetics of P-450 enzyme activity and protein expression at the same time. 7-Ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase activities were measured as an expression of the cytochrome P-450 enzyme activities. Both cell lines began to express these enzyme activities as early as 12h after infection. The activities increased linearly up to the 24 h time point, and were kept for 36 h. Western immunoblot analysis showed that these cytochrome P-450 proteins were detected at 16 h and reached maximum quantity at 24 h after infection. These data showed a good correlation between cytochrome P-450 enzyme activity and protein concentration throughout the process of P-450 gene expression by vaccinia virus vector, suggesting a complete formation of cytochrome P-450 holoenzyme from the early stage of the protein expression.

キーワード
cytochrome P-450
vaccinia virus
kinetics
Amo Type
Article
発行日
1993-12
出版物タイトル
Acta Medica Okayama
47巻
6号
出版者
Okayama University Medical School
開始ページ
377
終了ページ
382
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT