JaLCDOI 10.18926/AMO/31022
フルテキストURL fulltext.pdf
著者 Masuoka, Noriyoshi| Ubuka, Toshihiko| Kinuta, Masahiro| Yoshida, Shigeko| Taguchi, Tazuko|
抄録 <p>A new gas chromatographic method for the determination of sulfate was developed. In this method, sulfate was quantitatively converted to a volatile derivative, dimethyl sulfate, by a two-step procedure. First, sulfate was converted to silver sulfate by reaction with silver oxide, and then to dimethyl sulfate by reaction with methyl iodide. The derivative was analyzed by gas chromatography. Methyl methanesulfonate was used as an internal standard. The method was applied to the determination of total urinary sulfate. Phosphate and chloride ions, which interfered with the present method, were eliminated with the use of basic magnesium carbonate and an excess of silver oxide, respectively. Recovery was over 96% when 5 to 40 mumol/ml of sulfate was added to human urine samples.</p>
キーワード gas chromatography sulfate determination dimethy1 sulfate sulfuric acid urinary sulfate
Amo Type Article
発行日 1988-10
出版物タイトル Acta Medica Okayama
42巻
5号
出版者 Okayama University Medical School
開始ページ 247
終了ページ 252
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3223336
Web of Sience KeyUT A1988Q771900001
JaLCDOI 10.18926/AMO/30456
フルテキストURL fulltext.pdf
著者 Taguchi, Tazuko| Akagi, Reiko| Ubuka, Toshihiko|
抄録 <p>&lt;p&gt;Tissue contents and urinary excretion of taurine were studied in rats after the administration of L-cysteine and its derivatives. Average taurine content in the liver of rats fed a 25% casein diet for 7 days increased 2-fold 2h after the intraperitoneal administration of 5 mmol of L-cysteine per kg of body weight, whereas that in rats fed a 5% casein diet for 2 days increased only slightly. The difference in the liver taurine contents between these two groups was discussed in relation to cysteine dioxygenase. Taurine contents in the heart, brain and blood did not differ significantly between these two groups or between the control and the group of rats which received L-cysteine. The increase in liver taurine concentrations after L-cysteine administration was much higher than that after L-cystine administration, suggesting a difference in their absorption. The intraperitoneal administration of 5 mmol/kg of L-2-oxothiazolidine-4-carboxylate (OTCA) resulted in a 3-fold increase in liver taurine content. The average increase in taurine excretion in the 24-h urine after OTCA administration corresponded to about 6.0% and that in the next 24-h urine to about 2.6% of OTCA administered, suggesting that nearly 10% of OTCA was metabolized to taurine and excreted in the urine.&lt;/p&gt;</p>
キーワード taurine cysteine metabolism 2-oxothiazolidine-4-carboxyylate
Amo Type Article
発行日 1990-06
出版物タイトル Acta Medica Okayama
44巻
3号
出版者 Okayama University Medical School
開始ページ 123
終了ページ 128
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2382577
Web of Sience KeyUT A1990DM18300002