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ID 30136
フルテキストURL
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著者
Kudo, Chikako Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Suzuki, Tomoko Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Fukuoka, Sumie Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Asai, Shuta Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Suenaga, Hiroko Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Sasabe, Michiko Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Takano, Yoshitaka
Okuno, Tetsuro Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Toyoda, Kazuhiro Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Shiraishi, Tomonori Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Ichinose, Yuki Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
Inagaki, Yoshi-Shige Laboratory of Plant Pathology & Genetic Engineering, Faculty of Agriculture, Okayama University
抄録

Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY- 2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase- promoting complex or cyclosome ( APC/ C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus:: Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.

備考
Published with permission from the copyright holder.
This is published in Molecular Plant Pathology, 2007 Vol.8 Issue.4 pp.365-373
Publisher URL: http://dx.doi.org/10.1111/J.1364-3703.2007.00397.X
Direct access to Thomson Web of Science record
(C)2008 by John Wiley & Sons, Inc.
発行日
2007-07-01
出版物タイトル
Molecular Plant Pathology
8巻
4号
開始ページ
365
終了ページ
373
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
査読
有り
DOI
Web of Sience KeyUT
Submission Path
plant_sciences/3