フルテキストURL SciRep_7_1_7826.pdf
著者 Takatani, Shogo| Ozawa, Shinichiro| Yagi, Noriyoshi| Hotta, Takashi| Hashimoto, Takashi| Takahashi, Yuichiro| Takahashi, Taku| Motose, Hiroyasu|
抄録  Plant cortical microtubules align perpendicular to the growth axis to determine the direction of cell growth. However, it remains unclear how plant cells form well-organized cortical microtubule arrays in the absence of a centrosome. In this study, we investigated the functions of Arabidopsis NIMA-related kinase 6 (NEK6), which regulates microtubule organization during anisotropic cell expansion. Quantitative analysis of hypocotyl cell growth in the nek6-1 mutant demonstrated that NEK6 suppresses ectopic outgrowth and promotes cell elongation in different regions of the hypocotyl. Loss of NEK6 function led to excessive microtubule waving and distortion, implying that NEK6 suppresses the aberrant cortical microtubules. Live cell imaging showed that NEK6 localizes to the microtubule lattice and to the shrinking plus and minus ends of microtubules. In agreement with this observation, the induced overexpression of NEK6 reduced and disorganized cortical microtubules and suppressed cell elongation. Furthermore, we identified five phosphorylation sites in β-tubulin that serve as substrates for NEK6 in vitro. Alanine substitution of the phosphorylation site Thr166 promoted incorporation of mutant β-tubulin into microtubules. Taken together, these results suggest that NEK6 promotes directional cell growth through phosphorylation of β-tubulin and the resulting destabilization of cortical microtubules.
キーワード Cell growth Microtubules Plant cytoskeleton
備考 Published in Scientific Reports. 7, 7826(2017)
発行日 2017-08-10
出版物タイトル Scientific Reports
7巻
出版者 Nature Publishing Group
開始ページ 7826
ISSN 2045-23
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 28798328
DOI 10.1038/s41598-017-08453-5
Web of Sience KeyUT 000407400500058
関連URL https://doi.org/10.1038/s41598-017-08453-5
JaLCDOI 10.18926/AMO/54813
フルテキストURL 70_6_493.pdf
著者 Yasuhara, Takao| Kuwahara, Ken| Sasada, Susumu| Toyoshima, Atsuhiko| Morimoto, Jun| Kin, Kyohei| Manabe, Hiroaki| Miyoshi, Yasuyuki| Kusumegi, Akira| Takahashi, Yuichiro| Ito, Kiyoshi| Date, Isao|
抄録 Unexpected injuries can have a profound effect on a surgeonʼs performance and thus on patients and surgical departments. Here we describe a technique for performing surgery in the standing position, as done by a surgeon with an Achilles tendon rupture. During his prescribed 45-day non-weight-bearing period for the left ankle after surgery for an Achilles tendon rupture, the surgeon was able to participate in 15 surgeries as an operator or assistant, due to his use of a combination of injured-leg genuflection on a stool and a ʻSurgical Body Supportʼ device. Similarly injured surgeons may benefit from such support.
キーワード Achilles tendon rupture electromyogram genuflection injured leg standing position
Amo Type Short Communication
発行日 2016-12
出版物タイトル Acta Medica Okayama
70巻
6号
出版者 Okayama University Medical School
開始ページ 493
終了ページ 496
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2016 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 28003675