JaLCDOI 10.18926/AMO/31044
フルテキストURL fulltext.pdf
著者 Ueba, Osamu|
抄録 <p>Respiratory syncytial (RS) virus can be purified without losing its infectivity provided that each step of purification is carried out using NT buffer containing over 20% sucrose. Firstly, the virus grown on HES cells is efficiently removed from the culture fluid by precipitating with polyethylene glycol (PEG) 6,000, and the precipitate is suspended in a small amount of 20% sucrose-NT buffer, which results in about a 24-fold concentration of the original material. Then this suspension is centrifugated through 30% sucrose-NT buffer to obtain pellets, which are again suspended in 20% sucrose-NT buffer. This suspension is further centrifuged by discontinuous and linear sucrose density gradient. Finally, the specific infectivity of the purified virus was increased about 3,000-fold over that of the original material.</p>
キーワード respiratory syncytial virus purification
Amo Type Article
発行日 1978-08
出版物タイトル Acta Medica Okayama
32巻
4号
出版者 Okayama University Medical School
開始ページ 265
終了ページ 272
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 153087
NAID 120002312565
JaLCDOI 10.18926/AMO/30521
フルテキストURL fulltext.pdf
著者 Ueba, Osamu|
抄録 <p>Treatment of respiratory syncytial (RS) virus with Triton X-100 in solutions of low ionic strength solubilized not only glycoproteins but also some non-glycosylated proteins. Rate zonal sedimentation of the solubilized materials resulted in separation of the glycoproteins from the other components, i.e. one fraction predominantly composed of two glycoproteins each with molecular weight (mol. wt.) of approximately 100,000 and 53,000 was obtained. Electron microscopic observation of this fraction revealed numerous club- or rod-shaped fine structures, suggesting that these were spikes of RS virus. After removal of Triton X-100 the structures aggregated and formed oligomers and polymers.</p>
キーワード respiratory syncytial virus spike glycoproteins.
Amo Type Article
発行日 1980-09
出版物タイトル Acta Medica Okayama
34巻
4号
出版者 Okayama University Medical School
開始ページ 245
終了ページ 254
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6452027
Web of Science KeyUT A1980KK16800003