JaLCDOI 10.18926/AMO/30897
フルテキストURL fulltext.pdf
著者 Ikeda, Takahiro| Ubaka, Toshihiko| Ishino, Kazushi|
抄録 <p>The isoelectric point (pI) value of 3-mercaptopyruvate sulfurtransferase (MST) from human erythrocytes was determined to be 6.3 at 10 degrees C by isoelectric focusing in horizontal slab polyacrylamide gel containing 2% carrier ampholyte (pH 3-10). The value was determined by comparison with the electrofocused bands of bovine pancreatic ribonuclease A-glutathione mixed disulfides (RNase-SG), which were composed of 8 species containing 1 (RNase-SG1) through 8 (RNase-SG8) moles of glutathione per mole of ribonuclease A with different pI values ranging from 5.3 (RNase-SG8) to 8.8 (RNase-SG1). The pI value of the same enzyme in a 110,000 X g supernatant of rat liver was 5.9, which was the same as that of rat erythrocyte enzyme. Treatments of rat hemolysate with oxidized glutathione or diamide resulted in a shift of the pI of MST to a lower value, 5.7-5.5. This shift was inhibited when these treatments were performed in the presence of dithiothreitol. These results indicate that the treatment of the enzyme with oxidized glutathione results in the formation of enzyme-glutathione mixed disulfide.</p>
キーワード 3-mercaptopyruvate sulfurtransferase isoelectric point glutathione mixed disulfide isoelectric focusing
Amo Type Article
発行日 1989-02
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 1
終了ページ 7
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 2718769
Web of Science KeyUT A1989T938500001