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ID 57783
フルテキストURL
著者
Werner, Rudolf A. The Russell H. Morgan Department of Radiology and Radiological Science, Division of Nuclear Medicine and Molecular Imaging, Johns Hopkins School University of Medicine
Wakabayashi, Hiroshi Department of Nuclear Medicine, Hannover Medical School
Chen, Xinyu Department of Nuclear Medicine, Hannover Medical School
Hayakawa, Nobuyuki Department of Nuclear Medicine, Hannover Medical School
Lapa, Constantin Department of Nuclear Medicine, University Hospital, University of Würzburg
Rowe, Steven P. The Russell H. Morgan Department of Radiology and Radiological Science, Division of Nuclear Medicine and Molecular Imaging, Johns Hopkins School University of Medicine
Javadi, Mehrbod S. The Russell H. Morgan Department of Radiology and Radiological Science, Division of Nuclear Medicine and Molecular Imaging, Johns Hopkins School University of Medicine
Robinson, Simon Lantheus Medical Imaging
Higuchi, Takahiro Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences ORCID Kaken ID researchmap
抄録
We aimed to determine a detailed regional ventricular distribution pattern of the novel cardiac nerve PET radiotracer F-18-LMI1195 in healthy rabbits. Ex-vivo high resolution autoradiographic imaging was conducted to identify accurate ventricular distribution of F-18-LMI1195. In healthy rabbits, F-18-LMI1195 was administered followed by the reference perfusion marker Tl-201 for a dual-radiotracer analysis. After 20 min of F-18-LMI1195 distribution time, the rabbits were euthanized, the hearts were extracted, frozen, and cut into 20-mu m short axis slices. Subsequently, the short axis sections were exposed to a phosphor imaging plate to determine F-18-LMI1195 distribution (exposure for 3 h). After complete F-18 decay, sections were re-exposed to determine Tl-201 distribution (exposure for 7 days). For quantitative analysis, segmental regions of Interest (ROIs) were divided into four left ventricular (LV) and a right ventricular (RV) segment on mid-ventricular short axis sections. Subendocardial, midportion, and subepicardial ROIs were placed on the LV lateral wall. F-18-LMI1195 distribution was almost homogeneous throughout the LV wall without any significant differences in all four LV ROIs (anterior, posterior, septal and lateral wall, 99 +/- 2, 94 +/- 5, 94 +/- 4 and 97 +/- 3%LV, respectively, n.s.). Subepicardial Tl-201 uptake was significantly lower compared to the subendocardial portion (subendocardial, midportion, and subepicardial activity: 90 +/- 3, 96 +/- 2 and *80 +/- 5%LV, respectively, *p < 0.01 vs. midportion). This was in contradistinction to the transmural wall profile of F-18-LMI1195 (90 +/- 4, 96 +/- 5 and 84 +/- 4%LV, n.s.). A slight but significant discrepant transmural radiotracer distribution pattern of Tl-201 in comparison to F-18-LMI1195 may be a reflection of physiological sympathetic innervation and perfusion in rabbit hearts.
発行日
2019-11-19
出版物タイトル
Scientific Reports
9巻
出版者
Nature Publishing Group
開始ページ
17026
ISSN
2045-2322
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© Te Author(s) 2019
論文のバージョン
publisher
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DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1038/s41598-019-53596-2
ライセンス
https://creativecommons.org/licenses/by/4.0/