フルテキストURL Reprod_Fertil_Dev_28_6_673.pdf
著者 Yamamoto, Yuki| Kohka, Misa| Kobayashi, Yoshihiko| Woclawek-Potocka, Izabela| Okuda, Kiyoshi|
抄録 Endothelin (EDN) is a possible regulating factor of oviductal motility, which is important for the transport of gametes and embryo. To clarify the factors that control the secretion of EDN in the bovine oviduct, the expression of EDNs, EDN-converting enzymes (ECEs) and EDN receptors (EDNRs) were investigated. All isoforms of EDN (EDN1-3), ECE (ECE1 and ECE2) and EDNR (EDNRA and EDNRB) were immunolocalised in the epithelial cells of the ampulla and the isthmus. EDNRs were also immunolocalised in smooth-muscle cells. The mRNA expression of EDN2 and ECE2 was higher in cultured ampullary oviductal epithelial cells than in isthmic cells. The expression of EDN1, EDN2 and ECE2 in the ampullary tissue was highest on the day of ovulation. Oestradiol-17β increased EDN2 and ECE1 expression, while progesterone increased only ECE1 expression in cultured ampullary epithelial cells. These results indicate that EDNs are produced by epithelial cells and their target site is smooth-muscle and epithelial cells, and suggest that ovarian steroids are regulators of endothelin synthesis in ampullary oviductal epithelial cells.
キーワード endothelin converting enzyme endothelin receptor epithelial cell ovarian steroids oviductal contraction and relaxation
備考 This is an Accepted Manuscript of an article published by CSIRO Publishing
発行日 2014-11
出版物タイトル Reproduction, Fertility and Development
28巻
6号
出版者 CSIRO Publishing
開始ページ 673
終了ページ 681
ISSN 1031-3613
NCID AA10718189
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン author
PubMed ID 25370848
DOI 10.1071/RD14076
Web of Sience KeyUT 000374546100003
関連URL isVersionOf https://doi.org/10.1071/RD14076