JaLCDOI 10.18926/AMO/32850
フルテキストURL fulltext.pdf
著者 Wu, Yun-Feng| Matsuo, Noritaka| Sumiyoshi, Hideaki| Yoshioka, Hidekatsu|
抄録 <p>The purpose of this study was to clarify the mechanism responsible for the transcriptional regulation of the mouse Col5a3 gene in osteoblastic cells. Transient transfection into rat osteosarcoma ROS17/2.8 cells demonstrated that a region from nucleotides 337 to 1 was involved in the transcriptional activity of the Col5a3 gene. An electrophoretic mobility shift assay showed that Sp1/Sp3 and CBF/NF-Y bound to a GC-rich domain (194/186) and a CCAAT box (134/130) in the Col5a3 gene, respectively. Introduction of mutations or deletion into a GC-rich domain, the CCAAT box, or both elements decreased the transcription activity. Overexpression of Sp1 increases the transcription activity and interferes with Sp family binding to the GC-rich domain to decrease promoter activity. Therefore, the transcription of the mouse Col5a3 gene is cooperatively regulated by Sp1 and CBF/NF-Y in osteoblastic cells.</p>
キーワード type V collagen Sp1 CBF/NF-Y gene regulation osteoblastic cell
Amo Type Original Article
発行日 2010-04
出版物タイトル Acta Medica Okayama
出版者 Okayama University Medical School
開始ページ 95
終了ページ 108
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 20424664
Web of Sience KeyUT 000276996900003