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ID 48964
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Thumnail 66_5_399.pdf 2.91 MB
著者
Jing, Shao-Wu Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
Wang, Ya-Di Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
Kuroda, Masahiro Department of Radiation Technology, Graduate School of Health Sciences, Okayama University
Su, Jing-Wei Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
Sun, Guo-Gui Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
Liu, Qing Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
Cheng, Yun-Jie Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
Yang, Cong-Rong Department of Radiotherapy, the Fourth Hospital of Hebei Medical University
抄録
Hypoxia-inducible factor-1α (HIF-1α) has been found to enhance tumor invasion and metastasis, but no study has reported its action in esophageal carcinoma. The goal of this study was to explore the probable mechanism of HIF-1α in the invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo. mRNA and protein expression of HIF-1α, E-cadherin and matrix metalloproteinase-2 (MMP-2) under hypoxia were detected by RT-PCR and Western blotting. The effects of silencing HIF-1α on E-cadherin, MMP-2 mRNA and protein expression under hypoxia or normoxia were detected by RT-PCR and Western blotting, respectively. The invasive ability of Eca109 cells was tested using a transwell chambers. We established an Eca109-implanted tumor model and observed tumor growth and lymph node metastasis. The expression of HIF-1α, E-cadherin and MMP-2 in xenograft tumors was detected by Western blotting. After exposure to hypoxia, HIF-1α protein was up-regulated, both mRNA and protein levels of E-cadherin were down-regulated and MMP-2 was up-regulated, while HIF-1α mRNA showed no significant change. SiRNA could block HIF-1α effectively, increase E-cadherin expression and inhibit MMP-2 expression. The number of invading cells decreased after HIF-1α was silenced. Meanwhile, the tumor volume was much smaller, and the metastatic rate of lymph nodes and the positive rate were lower in vivo. Our observations suggest that HIF-1α inhibition might be an effective strategy to weaken invasion and metastasis in the esophageal carcinoma Eca109 cell line.
キーワード
hypoxia-inducible factor-1α
esophageal carcinoma
invasion and metastasis
Amo Type
Original Article
発行日
2012-10
出版物タイトル
Acta Medica Okayama
66巻
5号
出版者
Okayama University Medical School
開始ページ
399
終了ページ
407
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
著作権者
CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT