JaLCDOI 10.18926/AMO/30665
フルテキストURL fulltext.pdf
著者 Yoshida, Haruhiko| Akeho, Masahiro| Orido, Takashi| Yumoto, Tokichi| Furuse, Kiyowo| Maeyama, Iwao| Tanaka, Toshio|
抄録 <p>A total of 19 cases with bone tumors, including six osteosarcomas. three giant cell tumors of bone, one malignant fibrous histiocytoma, four nonossifying fibromas, four chondromas and one chondrosarcoma, were examined as to enzyme histochemistry; the enzymes consisted of alkaline phosphatase (ALPase), acid phosphatase (ACPase), nonspecific esterase (NSE), adenosine triphosphatase (ATPase), 5'-nucleotidase (5'-Nucl) and beta-glucuronidase (beta-Gl). Osteosarcoma was strongly positive for ALPase followed by 5'-Nucl. Giant cell tumor, malignant fibrous histiocytoma and nonossifying fibroma showed enzyme histochemistry similar to each other: multinucleated giant cells and round cells in these tumors were strongly positive for ACPase, NSE, ATPase and 5'-Nucl simulating osteoclasts and histiocytes, whereas spindle cells were positive for ATPase and 5'-Nucl in their cytoplasm and weakly positive for ACPase. Chondroma and chondrosarcoma were focally positive for ACPase and NSE; the ACPase was sensitive to tartaric acid treatment. These observations showed that ALPase activity is very characteristic to osteosarcoma, and is useful for its diagnosis. From enzyme histochemistry, giant cell tumor, malignant fibrous histiocytoma and nonossifying fibroma can be regarded as a histiocyte-derived tumor of bone in contrast to osteosarcoma and cartilaginous tumors.</p>
キーワード bone tumors enzyme histochemistry ultrastructure tissue culture histiocytes
Amo Type Article
発行日 1982-12
出版物タイトル Acta Medica Okayama
36巻
6号
出版者 Okayama University Medical School
開始ページ 469
終了ページ 482
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6297258
Web of Science KeyUT A1982PW07500006
JaLCDOI 10.18926/AMO/30542
フルテキストURL fulltext.pdf
著者 Miyamoto, Kanji| Miyano, keiko| Tabuchi, Kazuo| Nishimoto, Akira| Yoshida, Haruhiko| Yumoto, Tokichi| Tanaka, Toshio|
抄録 Chromosome studies of a malignant lymphoma cell line derived from the brain were made by Q- and G-banding techniques. The modal number of chromosomes was 45. Complex structural rearrangements were present, but the 14q+ marker chromosome frequently seen in malignant lymphomas was not identified in the cell line. The main karyotype in cells analyzed was 45, X, -Y, del (2) (q21q23), t (3;?) (p25;?), t (p12;?), -8, 11q+, 18q+, +mar. Absence of the 14q+ may be explained by: firstly, clones which possessed 14q+ marker chromosome in brain tumor cells may have been selected out with increasing culture time and repeated passages; or secondly, the presence of the 14q+ marker chromosome depends on the type of lymphoma.
キーワード brain lymphoma tissue culture cell line chromosome negative 14q+.
Amo Type Brief Note
発行日 1980-04
出版物タイトル Acta Medica Okayama
34巻
2号
出版者 Okayama University Medical School
開始ページ 117
終了ページ 122
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6450513
Web of Science KeyUT A1980JY15600005
JaLCDOI 10.18926/AMO/30532
フルテキストURL fulltext.pdf
著者 Yumoto, Tokichi| Yoshida, Haruhiko| Ando, Kazufumi| Tanaka, Toshio|
抄録 <p>A transplantable plasma cell leukemia cell line, designated as S27, was established from a 12-month-old female New Zealand Black mouse (NZB); serum showed polyclonal elevation of gamma globulin with agarose-agar gel electrophoresis. Immunoelectrophoretic analysis of the serum showed precipitation lines in the IgG1, IgG2a, IgG2b and IgM regions. The amount of serum immunoglobulin increased rapidly with tumor growth. S27 cells proliferating in the spleen contained simultaneously IgG1, IgG2a, IgG2b in the cytoplasm as revealed by indirect immunofluorescence. Membrane immunofluorescence revealed IgG1 on the tumor cell surface. S27 cells were transplantable to syngeneic NZB mice with inoculation of spleen cell suspension, and showed the same histological and immunological findings as those of the original mouse. These findings imply that a single clone of plasma cells has the capacity to produce more than one class of immunoglobulin.</p>
キーワード polyclonal gammopathy plasma cell leukemia mouse
Amo Type Article
発行日 1980-12
出版物タイトル Acta Medica Okayama
34巻
6号
出版者 Okayama University Medical School
開始ページ 389
終了ページ 399
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 6451145
Web of Science KeyUT A1980KZ17800005