start-ver=1.4
cd-journal=joma
no-vol=19
cd-vols=
no-issue=2-3
article-no=
start-page=452
end-page=461
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2018
dt-pub=20180806
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Laser-Induced Fluorometry for Capillary Electrophoresis
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.
en-copyright=
kn-copyright=

en-aut-name=KanetaTakashi
en-aut-sei=Kaneta
en-aut-mei=Takashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=

affil-num=1
en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University
kn-affil=
en-keyword=DNA sequencing
kn-keyword=DNA sequencing
en-keyword=capillary electrophoresis
kn-keyword=capillary electrophoresis
en-keyword=laser-induced fluorescence
kn-keyword=laser-induced fluorescence
en-keyword=micellar electrokinetic chromatography
kn-keyword=micellar electrokinetic chromatography
END