ID | 16507 |
Eprint ID | 16507
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フルテキストURL | |
タイトル(別表記) | Detection of antibodies to denatured DNA by radioimmunoassay Part I: Comparison of methods
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著者 |
更井 哲夫
岡山大学第三内科
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抄録 | (14)C•denatured(d-) DNA, (125)I•d-DNA and (3)H•Actinomycin D•d-DNA were used as radioabelled DNA antigens for radioimmunoassay of antibody to d-DNA. The (14)C-DNA derived rom E. Coli was the most suitable antigen for simultaneous detection of antibodies to native n-) DNA and d-DNA. (125)I•d-DNA was easily prepared by iodination of heat denatured alf thymus DNA in vitro. (3)H•Actinomycin D•d-DNA was also easily prepared by mixing I-DNA with (3)H•Actinomycin D in vitro. Using normal human sera and systemic lupus eryhematosus (SLE) sera that had contained antibody to d-DNA, half saturated ammonium sulfate method and filter method were employed to separate immunoglobulin-bound antigen from free antigen. To avoid nonimmunological bindings to d-DNA, pH and ionic strength of assay system were determined as 8.3 and 0.15 respectively. In the filter method, application of sonication to radiolabelled d-DNA prevented nonspecific binding of the antigen to the filter nembrane. However, in the half saturated ammonium sulfate method, d-DNA binding between SLE sera and sonicated radiolabelled d-DNA decreased markedly. When (3)H•Actinomycin D•d-DNA was the antigen, neither the half saturated ammonium sulfate method nor the filter method was suitable for evaluation of antibody to d-DNA. There was fairly good correlation between the half saturated ammonium sulfate method and the filter method, using either (14)C•d-DNA or (125)I•d-DNA as the antigen. The half saturated ammonium sulfate method and the filter method were reproducible, quantitative, sensitive and specific enough to detect antibody to d-DNA.
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発行日 | 1981-02-28
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 93巻
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号 | 1-2号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 165
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終了ページ | 177
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/93/1-2/93_1-2_165/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/index.html
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言語 |
Japanese
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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