このエントリーをはてなブックマークに追加
ID 16513
Eprint ID
16513
フルテキストURL
Thumnail 93_179.pdf 612 KB
タイトル(別表記)
Detection of antibodies to denatured DNA by radioimmunoassay Part II: Clinical significance in sera of patients with systemic rheumatic diseases and other non-rheumatic diseases
著者
更井 哲夫 岡山大学第三内科
抄録
Antibodies to native (n-) DNA and denatured (d-) DNA were detected simultaneously and quantitatively in the sera of patients with systemic lupus erythematosus (SLE) and other rheumatic diseases by a filter method using (14)C•n-DNA or (14)C•d-DNA as the radiolabelled antigen. In a group of patients with SLE, most of the sera had antibodies to both n-DNA and d-DNA, only a few sera had antibody to d-DNA alone. In contrast, none of the sera reacted with n-DNA alone. On the other hand, sera from patients with progressive systemic scleroderma, dermatomyositis, polymyositis or SjΦgren's syndrome had antibody to d-DNA alone. In order to estimate the participation of anti-d-DNA antibody in lupus nephritis, patients with SLE were classified into two groups according to immunofluorescent glomerular staining. In the group of patients that had lumpy or granular staining, the sera reacted predominantly with n-DNA. In contrast, sera from the other group of patients that had mesangial or linear staining had antibodies that mainly reacted with d-DNA. The difference in reactivity between these two groups was statistically significant (p 0.02). In the other group of sera from patients with non-rheumatic diseases, anti-d-DNA antibodies were detected by the half saturated ammonium sulfate method using (125)I•d-DNA as the antigen. Positive incidences of anti-d-DNA antibody in these group were as follows: lung tuberculosis 21%, Graves' disease 55%, Hashimoto's thyroiditis 73%, chronic hepatitis 52%, cancer patients 25%, and patients undergoing hemodialysis 24%. Significant correlation between antinuclear antibody and anti-d-DNA antibody was seen in a group of patients with Hashimoto's thyroiditis, in patients undergoing hemodialysis, and in lung tuberculosis. In patients with chronic hepatitis, significant correlation was seen between HBsAg and anti-dDNA antibody. These results suggest that the anti-d-DNA antibody has an extremely wide distribution in rheumatic and non-rheumatic diseases with the highest titer and incidence in SLE. In SLE sera, however, anti-d-DNA antibody is less relevant to the severity of lupus nephritis and has a close correlation to anti-n-DNA antibody.
発行日
1981-02-28
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
93巻
1-2号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
179
終了ページ
190
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/93/1-2/93_1-2_179/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma