フルテキストURL |
NatCommun_12501.pdf
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著者 |
Ohtsuki, Takashi|
Kanzaki, Shigeto|
Nishimura, Sae|
Kunihiro, Yoshio|
Sisido, Masahiko|
Watanabe, Kazunori|
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抄録 |
The possibility of spatiotemporally photocontrolling translation holds considerable promise for studies on the biological roles of local translation in cells and tissues. Here we report caged aminoacyl-tRNAs (aa-tRNAs) synthesized using a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-cage compound. DEACM-caged aa-tRNA does not spontaneously deacylate for at least 4 h in neutral aqueous solution, and does not bind to the elongation factor Tu. On irradiation at ∼405 nm at 125 mW cm(-2), DEACM-aa-tRNA is converted into active aa-tRNA with a half-life of 19 s. Notably, this rapid uncaging induced by visible light does not impair the translation system. Translation is photoinduced when DEACM-aa-tRNA carrying a CCCG or a CUA anticodon is uncaged in the presence of mRNAs harbouring a CGGG four-base codon or a UAG amber codon, respectively. Protein synthesis is phototriggered in several model systems, including an in vitro translation system, an agarose gel, in liposomes and in mammalian cells.
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キーワード |
Molecular engineering
Optogenetics
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備考 |
This is an article published by Nature Publishing Group
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発行日 |
2016-08
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出版物タイトル |
Nature Communications
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巻 |
7巻
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開始ページ |
12501
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ISSN |
2041-1723
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NCID |
AA12645905
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資料タイプ |
学術雑誌論文
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言語 |
English
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OAI-PMH Set |
岡山大学
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著作権者 |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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論文のバージョン |
publisher
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PubMed ID |
27530762
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DOI |
10.1038/ncomms12501
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Web of Sience KeyUT |
000381771700001
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関連URL |
https://doi.org/10.1038/ncomms12501
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