著者 佃 和憲| 浅野 博昭| 万代 康弘| 藤原 俊義|
発行日 2016-08-01
出版物タイトル 岡山医学会雑誌
128巻
2号
資料タイプ 学術雑誌論文
著者 Hayashi, Tatsuro| Asano, Hiroaki| Toyooka, Shinichi| Tsukuda, Kazunori| Soh, Junichi| Shien, Tadahiko| Taira, Naruto| Maki, Yuho| Tanaka, Norimitsu| Doihara, Hiroyoshi| Nasu, Yasutomo| Huh, Nam-ho| Miyoshi, Shinichiro|
発行日 2012-05
出版物タイトル Journal of Cancer Research and Clinical Oncology
138巻
5号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/52140
フルテキストURL 68_1_23.pdf
著者 Ueno, Tsuyoshi| Toyooka, Shinichi| Fukazawa, Takuya| Kubo, Takafumi| Soh, Junichi| Asano, Hiroaki| Muraoka, Takayuki| Tanaka, Norimitsu| Maki, Yuho| Shien, Kazuhiko| Furukawa, Masashi| Sakaguchi, Masakiyo| Yamamoto, Hiromasa| Tsukuda, Kazunori| Miyoshi, Shinichiro|
抄録 The microRNA-34s (miR-34s) have p53 response elements in their 5ʼ-flanking regions and demonstrate tumor-suppressive functions. In malignant pleural mesothelioma (MPM), we previously reported that expression of miR-34b and miR-34c (miR-34b/c) was frequently downregulated by methylation in MPM cell lines and primary tumors. The forced overexpression of miR-34b/c showed significant antitumor effects with the induction of apoptosis in MPM cells. In this study, we examined the in vivo antitumor effects of miR-34b/c using adenovirus vector on MPM. We subcutaneously transplanted NCI-H290, a human MPM cell line, into BALB/C mice and injected adenovirus vector expressing miR-34b/c, luciferase driven by the cytomegalovirus promoter (Ad-miR-34b/c or Ad-Luc), or PBS control into tumors over 5mm in diameter. A statistically significant growth inhibition of the tumor volume was observed in the Ad-miR-34b/c group from day 6 onward compared to the Ad-Luc group. The inhibition rate of Ad-miR-34b/c, compared to the tumor volume treated with Ad-Luc, was 58.6% on day 10 and 54.7% on day13. Our results indicate that adenovirus-mediated miR-34b/c gene therapy could be useful for the clinical treatment of MPM.
キーワード mesothelioma microRNA microRNA-34b/c p53
Amo Type Original Article
発行日 2014-02
出版物タイトル Acta Medica Okayama
68巻
1号
出版者 Okayama University Medical School
開始ページ 23
終了ページ 26
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2014 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol68/iss1/4
PubMed ID 24553485
Web of Sience KeyUT 000331592800004
著者 Muraoka, Takayuki| Soh, Junichi| Toyooka, Shinichi| Aoe, Keisuke| Fujimoto, Nobukazu| Hashida, Shinsuke| Maki, Yuho| Tanaka, Norimitsu| Shien, Kazuhiko| Furukawa, Masashi| Yamamoto, Hiromasa| Asano, Hiroaki| Tsukuda, Kazunori| Kishimoto, Takumi| Otsuki, Takemi| Miyoshi, Shinichiro|
発行日 2013-12
出版物タイトル Lung Cancer
82巻
3号
資料タイプ 学術雑誌論文
著者 Maki, Yuho| Soh, Junichi| Ichimura, Kouichi| Shien, Kazuhiko| Furukawa, Masashi| Muraoka, Takayuki| Tanaka, Norimitsu| Ueno, Tsuyoshi| Yamamoto, Hiromasa| Asano, Hiroaki| Tsukuda, Kazunori| Toyooka, Shinichi| Miyoshi, Shinichiro|
発行日 2013-01
出版物タイトル Oncology Reports
29巻
1号
資料タイプ 学術雑誌論文
著者 Ueno, Tsuyoshi| Tsukuda, Kazunori| Toyooka, Shinichi| Ando, Midori| Takaoka, Munenori| Soh, Junichi| Asano, Hiroaki| Maki, Yuho| Muraoka, Takayuki| Tanaka, Norimitsu| Shien, Kazuhiko| Furukawa, Masashi| Yamatsuji, Tomoki| Kiura, Katsuyuki| Naomoto, Yoshio| Miyoshi, Shinichiro|
発行日 2012-04
出版物タイトル Lung Cancer
76巻
1号
資料タイプ 学術雑誌論文
著者 Tanaka, Norimitsu| Toyooka, Shinichi| Soh, Junichi| Kubo, Takafumi| Yamamoto, Hiromasa| Maki, Yuho| Muraoka, Takayuki| Shien, Kazuhiko| Furukawa, Masashi| Ueno, Tsuyoshi| Asano, Hiroaki| Tsukuda, Kazunori| Aoe, Keisuke| Miyoshi, Shinichiro|
発行日 2012-04
出版物タイトル Lung Cancer
76巻
1号
資料タイプ 学術雑誌論文
著者 Shien, Kazuhiko| Toyooka, Shinichi| Ichimura, Kouichi| Soh, Junichi| Furukawa, Masashi| Maki, Yuho| Muraoka, Takayuki| Tanaka, Norimitsu| Ueno, Tsuyoshi| Asano, Hiroaki| Tsukuda, Kazunori| Yamane, Masaomi| Oto, Takahiro| Kiura, Katsuyuki| Miyoshi, Shinichiro|
発行日 2012-07
出版物タイトル Lung Cancer
77巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/49253
フルテキストURL 67_1_19.pdf
著者 Furukawa, Masashi| Soh, Junichi| Yamamoto, Hiromasa| Ichimura, Kouichi| Shien, Kazuhiko| Maki, Yuho| Muraoka, Takayuki| Tanaka, Norimitsu| Ueno, Tsuyoshi| Asano, Hiroaki| Tsukuda, Kazunori| Toyooka, Shinichi| Miyoshi, Shinichiro|
抄録 Nuclear factor of κ-light polypeptide gene enhancer in B cells inhibitor α (NFKBIA), which is a tumor suppressor gene, was found to be silenced in lung adenocarcinomas. We examined NFKBIA expression, mutations in the EGFR and K-ras genes, and EML4-ALK fusion in 101 resected lung adenocarcinoma samples from never-smokers. NFKBIA expression was evaluated using immunohistochemistry. NFKBIA expression was negative in 16 of the 101 samples (15.8%). EGFR and K-ras mutations and EML4-ALK fusion were detected in 61 (60.5%), 1 (1.0%), and 2 (2.0%) of the 101 samples, respectively, in a completely mutually exclusive manner. Negative NFKBIA expression was observed significantly more frequently among the tumors with none of the three genetic alterations compared to those with such alterations (p=0.009). In addition, negative NFKBIA expression was significantly more frequent among the EGFR-wild type samples compared to the EGFR-mutant samples (p=0.013). In conclusion, NFKBIA expression was silenced in adenocarcinomas without EGFR/K-ras mutations or EML4-ALK fusion, suggesting that the silencing of NFKBIA may play an important role in the carcinogenesis of adenocarcinomas independent of EGFR/K-ras mutations or EML4-ALK fusion.
キーワード never-smoker lung cancer adenocarcinoma nuclear factor of κ-light polypeptide gene enhancer in B cells inhibitor α epidermal growth factor receptor
Amo Type Original Article
発行日 2013-02
出版物タイトル Acta Medica Okayama
67巻
1号
出版者 Okayama University Medical School
開始ページ 19
終了ページ 24
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2013 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol67/iss1/3
PubMed ID 23439505
Web of Sience KeyUT 000316829900003
関連URL http://ousar.lib.okayama-u.ac.jp/metadata/52534
JaLCDOI 10.18926/AMO/48967
フルテキストURL 66_5_423.pdf
著者 Furukawa, Masashi| Izumi, Sadanobu| Tsukuda, Kazunori| Tokumo, Masaki| Sakurai, Jun| Mano, Shohey|
抄録 An 81-year-old man was found to have a pancreatic head tumor on abdominal computed tomography (CT) performed during a follow-up visit for sigmoid colon cancer. The tumor had a diameter of 35mm on the CT scan and was diagnosed as pancreatic head carcinoma T3N0M0. The patient was treated with pylorus-preserving pancreaticoduodenectomy. Histopathological examination showed that the tumor had grown within a hollow structure, was contiguous with a duodenal diverticulum, and had partially invaded the pancreas. Immunohistochemistry results were as follows:CK7 negative, CK20 positive, CD10 negative, CDX2 positive, MUC1 negative, MUC2 positive, MUC5AC negative, and MUC6 negative. The tumor was diagnosed as duodenal carcinoma from the duodenal diverticulum. Preoperative imaging showed that the tumor was located in the head of the pancreas and was compressing the common bile duct, thus making it appear like pancreatic cancer. To the best of our knowledge, this is the second report of a case of duodenal carcinoma from a duodenal diverticulum mimicking pancreatic carcinoma.
キーワード duodenal carcinoma duodenal diverticulum pancreatic carcinoma
Amo Type Case Report
発行日 2012-10
出版物タイトル Acta Medica Okayama
66巻
5号
出版者 Okayama University Medical School
開始ページ 423
終了ページ 427
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2012 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol66/iss5/7
PubMed ID 23093061
Web of Sience KeyUT 000310253900007
JaLCDOI 10.18926/AMO/46629
フルテキストURL 65_3_179.pdf
著者 Teramen, Hirotake| Tsukuda, Kazunori| Tanaka, Norimitsu| Ueno, Tsuyoshi| Kubo, Takafumi| Ando, Midori| Soh, Junichi| Asano, Hiroaki| Pass, Harvery I.| Toyooka, Shinichi| Miyoshi, Shinichiro|
抄録 Suppression of p21 has been implicated in the genesis and progression of many human malignancies. DNA methylation is an important mechanism of gene silencing in human malignancies. In this study, we examined the expression status and aberrant methylaion of p21 in lung cancers and malignant pleural mesotheliomas (MPM). We used 12 small cell lung cancer (SCLC) cell lines, 13 non-small cell lung cancer (NSCLC) cell lines, 50 primary NSCLCs, 6 MPM cell lines and 10 primary MPMs. The expression and methylation of p21 was examined by reverse transcription-PCR (RT-PCR), Western blotting and methylation-specific PCR (MSP) assay. Loss of p21 protein expression was observed in 7 SCLC cell lines (58.3%), 5 NSCLC cell lines (38.5%) and 3 MPM cell lines (50%) while mRNA expression was lost in 2 SCLC cell lines (16.7%), 2 NSCLC cell lines (15.4%) and none of the MPM cell lines. Aberrant methylation of p21 was found in 8.3% of SCLC cell lines, 30.2% of NSCLCs and 6.3% of MPMs. Among primary NSCLCs, methylation in adenocarcinomas was significantly more frequent than in squamous cell carcinomas. Loss of p21 expression was frequently observed in lung cancers and MPMs and aberrant methylation was one of the mechanisms of suppression of p21, especially in NSCLCs.
キーワード p21 methylation lung cancer mesothelioma
Amo Type Original Article
発行日 2011-06
出版物タイトル Acta Medica Okayama
65巻
3号
出版者 Okayama University Medical School
開始ページ 179
終了ページ 184
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 CopyrightⒸ 2011 by Okayama University Medical School
論文のバージョン publisher
査読 有り
Submission Path amo/vol65/iss3/4
PubMed ID 21709715
Web of Sience KeyUT 000292017500004
著者 Yano, Masaaki| Ouchida, Mamoru| Shigematsu, Hisayuki| Tanaka, Noriyoshi| Ichimura, Koichi| Kobayashi, Kazuyasu| Inaki, Yasuhiko| Toyooka, Shinichi| Tsukuda, Kazunori| Shimizu, Nobuyoshi| Shimizu, Kenji|
発行日 2004-10-20
出版物タイトル International Journal of Cancer
112巻
1号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/32909
フルテキストURL fulltext.pdf
著者 Tsukuda, Kazunori| Ikeda, Eiji| Takagi, Shoji| Miyake, Takayoshi| Muraoka, Takayuki| Watanabe, Keitaro| Hirai, Ryuji| Moriyama, Shigeharu| Nawa, Sugato| Kunitomo, Tadayoshi| Tsuji, Hisashi|
抄録 <p>Gastrointestinal stromal tumors (GISTs) have been reported to occasionally occur in patients with neurofi bromatosis type 1 (NF-1), and many cases have had multiple lesions predominantly involving the small intestine. We report herein a case of multiple GISTs associated with NF-1 from whom laparoscopic surgery was benefi cial. In a 79-year-old female admitted with anemia and melena, the abdominal computed tomography revealed a tumor arising from the small intestine. Laparoscopic surgery was performed, and another small tumor was revealed during laparoscopic observation. Extracorporeal partial and wedge resection of the small intestine were undertaken. Both lesions were diagnosed as typical GISTs of low risk. Laparoscopic surgery would be useful for examination and a minimally invasive approach to tumors of the small intestine, especially on cases with the possibility of multiple tumors.</p>
キーワード gastrointestinal stromal tumor neurofi bromatosis type 1 laparoscopic surgery
Amo Type Case Report
発行日 2007-02
出版物タイトル Acta Medica Okayama
61巻
1号
出版者 Okayama University Medical School
開始ページ 47
終了ページ 50
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol61/iss1/7
PubMed ID 17332842
Web of Sience KeyUT 000244432400007
JaLCDOI 10.18926/AMO/32891
フルテキストURL fulltext.pdf
著者 Yuasa, Ichiro| Tsukuda, Kazunori| Hirai, Ryuji| Ota, Tetsuya| Murakami, Masakazu| Naito, Minoru| Doihara, Hiroyoshi| Date, Hiroshi| Shimizu, Nobuyoshi|
抄録 <p>Resection is the only curative treatment for liver metastasis of colorectal cancers. Despite the supreme regenerative potential of the liver, major hepatectomy sometimes leads to liver failure, and the limitation of resectable liver volumes makes advanced tumors inoperable. This study was attempted to promote liver regeneration using hepatocyte growth factor (HGF) gene transfection by venous-administered adenovirus and to improve the survival of rats after massive hepatectomy. The adenovirus that encodes HGF was administered to rats before 85%-hepatectomy. The administration of HGF gene improved the survival of rats after massive hepatectomy, while the administration of control adenovirus deteriorated their survival. Gene transfection of HGF showed up-regulation of serum HGF, stimulation of hepatocellular proliferation and rapid liver regeneration. Moreover, HGF administration reduced apoptosis of hepatocytes. The administration of HGF gene prevented liver dysfunction after major hepatectomy and may be a new assist for surgery.</p>
キーワード gene therapy hepatectomy HGF adenoviral vector
Amo Type Original Article
発行日 2007-04
出版物タイトル Acta Medica Okayama
61巻
2号
出版者 Okayama University Medical School
開始ページ 81
終了ページ 88
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol61/iss2/5
PubMed ID 17471308
Web of Sience KeyUT 000245875600005
JaLCDOI 10.18926/AMO/32112
フルテキストURL fulltext.pdf
著者 Ota, Tetsuya| Hirai, Ryuji| Tsukuda, Kazunori| Murakami, Masakazu| Naitou, Minoru| Shimizu, Nobuyoshi|
抄録 <p>We report a case requiring biliary reconstruction with right hepatic lobectomy due to biliary strictures caused by continuous cholangitis after laparoscopic bile duct injury. The patient, a 55-year-old woman, underwent laparoscopic cholecystectomy for cholelithiasis at another hospital. Although a bile leakage from the intraabdominal drain was observed several days after the operation, the patient was not given adequate treatment to stop the leakage. Two months after the initial laparoscopic cholecystectomy, she was referred to our hospital. Endoscopic retrograde cholangiopancreatography (ERCP) showed complete obstruction of the common hepatic duct, which was caused by clipping during laparoscopic cholecystectomy. Cholangiography from percutaneous transhepatic biliary drainage (PTBD) catheters revealed that sections of the secondary branches of the right intrahepatic bile duct had become constricted due to persistent cholangitis. Fortunately, the left hepatic duct was judged to be normal by imaging. Therefore, we elected to perform a right hepatic lobectomy and left hepaticojejunostomy, because we felt that performing a hepaticojejunostomy without hepatic resection would put the patient at risk of continuing to suffer from cholangitis. The patient was discharged on the 55 th postoperative day, and, 5 years after reconstructive surgery, is healthy and has remained free from biliary strictures in the remnant liver. Appropriate decision-making is essential in the treatment of biliary injury after laparoscopic cholecystectomy. Surgeons should not hesitate to perform biliary reconstruction with hepatic resection to reduce the risk of cholangitis or biliary strictures of the remnant liver. More importantly, preoperative clear imaging of the biliary tree and suitable management of any biliary injury which might occur are necessary to avoid having to perform reconstructive surgery.</p>
キーワード ?biliary injury laparoscopic cholecystectomy hepatic resection
Amo Type Article
発行日 2004-06
出版物タイトル Acta Medica Okayama
58巻
3号
出版者 Okayama University Medical School
開始ページ 163
終了ページ 167
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol58/iss3/8
PubMed ID 15471439
Web of Sience KeyUT 000222273300008
JaLCDOI 10.18926/AMO/31828
フルテキストURL fulltext.pdf
著者 Tsukuda, Kazunori| Tsuji, Hisashi| Kunitomo, Tadayoshi| Aokage, Keiju| Miyake, Takayoshi| Nakahara, Saki| Masuda, Hiroko|
抄録 <p>Breast cancer with cartilaginous and/or osseous metaplasia is a type of metaplastic carcinomas and is a rare disease. We report the case of a 49 year-old female who underwent right mastectomy for a large breast tumor. Histological examinations revealed a mixed tumor with both stromal and epithelial elements;the stroma showed poor differentiated spindle-shape and multiform cells with a massive osseous matrix, and atypical epithelial cells, which mainly existed on the surface of the cysts, showed nucleic atypia. The tumor was diagnosed as a malignant phyllodes tumor with osteosarcomatous differentiation;it was not identified as a metaplastic carcinoma because of the lack of proof of a cancerous component. Two years after a mastectomy, swelling of the axillary lymph nodes was found and a biopsy was performed. Histological findings for the lymph node indicated a metastasis of the invasive ductal carcinoma. The primary tumor was re-examined and was considered to be the origin of the lymph nodal metastasis. Lymph nodal metastasis of cancer proved that the primary tumor had cancerous potential, and the pathological diagnosis was altered to a breast cancer with cartilaginous and/or osseous metaplasia.</p>
キーワード breast cancer metaplastic cancer phyllodes tumor
Amo Type Case Report
発行日 2009-12
出版物タイトル Acta Medica Okayama
63巻
6号
出版者 Okayama University Medical School
開始ページ 367
終了ページ 371
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol63/iss6/8
PubMed ID 20035293
Web of Sience KeyUT 000273145900008
JaLCDOI 10.18926/AMO/30953
フルテキストURL fulltext.pdf
著者 Tsukuda, Kazunori| Furutani, Shiro| Nakahara, Saki| Tada, Akihiro| Watanabe, Keitaro| Takagi, Shoji| Ikeda, Eiji| Hirai, Ryuji| Tsuji, Hisashi|
抄録 <p>Abscess formation of the round ligament of the liver is very rare. We report a case of a 70-year-old female with abscess of the round ligament after an endoscopic papillotomy for choledocholithiasis. On the 21st day following papillotomy, abscess formation of the round ligament was found by ultrasonographic examination. Surgical treatment was performed because conservative therapy was not effective. The purulent fluid and necrotic tissue at the round ligament were completely removed. Cultures obtained from the abscess grew Staphylococcus epidermidis, but the mechanism of abscess formation in this case remains unclear.</p>
キーワード round ligament of the liver abscess
Amo Type Case Report
発行日 2008-12
出版物タイトル Acta Medica Okayama
62巻
6号
出版者 Okayama University Medical School
開始ページ 411
終了ページ 413
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
論文のバージョン publisher
査読 有り
Submission Path amo/vol62/iss6/8
Web of Sience KeyUT 000262025000008
著者 佃 和憲|
発行日 2001-03-25
出版物タイトル
資料タイプ 学位論文