著者 別宮 洋子| 二宮 善文| 大橋 俊孝|
発行日 2012-04-01
出版物タイトル 岡山医学会雑誌
124巻
1号
資料タイプ 学術雑誌論文
著者 Demircan, Kadir| Hirohata, Satoshi| Nishida, Keiichiro| Hatipoglu, Omer F.| Oohashi, Toshitaka| Yonezawa, Tomoko| Apte, Suneel S.| Ninomiya, Yoshifumi|
発行日 2005-5
出版物タイトル Arthritis & Rheumatism
52巻
5号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/31831
フルテキストURL fulltext.pdf
著者 Hatipoglu, Omer Faruk| Hirohata, Satoshi| Yaykasli, Kursat Oguz| Cilek, Mehmet Zeynel| Demircan, Kadir| Shinohata, Ryoko| Yonezawa, Tomoko| Oohashi, Toshitaka| Kusachi, Shozo| Ninomiya, Yoshifumi|
抄録 <p>ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatory-induced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.</p>
キーワード ADAMTS1 gene regulation metalloproteinase
Amo Type Original Article
発行日 2009-04
出版物タイトル Acta Medica Okayama
63巻
2号
出版者 Okayama University Medical School
開始ページ 79
終了ページ 85
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 19404339
Web of Sience KeyUT 000265457600002
JaLCDOI 10.18926/AMO/31728
フルテキストURL fulltext.pdf
著者 Nomoto, Hiroyuki| Oohashi, Toshitaka| Hirakawa, Satoshi| Ueki, Yasuyoshi| Ohtsuki, Hiroshi| Ninomiya, Yoshifumi|
抄録 <p>We herein determined by fluorescence in situ hybridization the chromosomal localization of 2 human genes, BRAL1 and BCAN, both of which belong to the link-module superfamily, i.e. to the same band of chromosome 1q21-23. Further analysis of the genomic organization of BRAL1 and BCAN revealed that the BRAL1 gene was located 20-kb upstream of the BCAN start site. We isolated a polymorphic dinucleotide (CA) repeat sequence from a genomic clone containing the BCAN gene. High heterozygosity (0.79) makes this polymorphism a useful marker in the study of genetic disorders. Knowledge of the structure of the genes and the marker provides essential information for further analysis of the gene locus at chromosome 1q21-23.</p>
キーワード BRAL1 BCAN FISH schizophrenia polymorphic marker
Amo Type Article
発行日 2002-02
出版物タイトル Acta Medica Okayama
56巻
1号
出版者 Okayama University Medical School
開始ページ 25
終了ページ 29
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 11873941
Web of Sience KeyUT 000174031300005
著者 大橋 俊孝|
発行日 1992-03-28
出版物タイトル
資料タイプ 学位論文