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ID 13932
Eprint ID
13932
フルテキストURL
Thumnail 105_49.pdf 1.53 MB
タイトル(別表記)
Central nervous cells with strongly negatively-charged surface-coats
著者
村上 宅郎 岡山大学医学部第二解剖学教室
田口 勇仁 岡山大学医学部第二解剖学教室
大塚 愛二 岡山大学医学部第二解剖学教室
抄録
Light microscopy of tissue sections stained with cationic iron colloid (pH 1.0-2.0) and nuclear fast red or with this colloid and thionin revealed the presence of numerous neurons with a strong negative-charge or coated with sulfated proteoglycans such as chondroitin sulfates in the adult rat brain. These neurons were distributed mainly in the hippocampal subiculum, zona incerta, cerebral cortex (V lamina), medical cerebellar nuclei and certain other nuclei such as ventral pontine nuclei. In the hippocampal formation, the strongly negatively-charged cells seemed to be identical with the GABAergic inhibitory interneurons reactive to the lectin Vicia villosa agglutinin. The neurons, including the GABAergic Purkinje's cells, of the cerebellar cortex showed no reaction to the cationic iron colloid at pH 1.0-2.0. Many non-GABAergic large neurons of the ventral pontine nuclei were well reactive to the colloid at pH 1.0-2.0. These findings suggest that the cationic iron colloid at pH 1.0-2.0 mainly stains some subsets of GABA-ergic neurons, and additionally stains some non-GABAergic interneurons projecting long association or commissural fibers.
キーワード
Cationic iron colloid staining
central nervous system
neurons
sulfated proteoglycans
発行日
1993-02-27
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
105巻
1-2号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
49
終了ページ
53
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/105/1-2/105_1-2_49/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
参考文献
1) Murakami T, Taguchi T,Ohtsuka A, Sano K, Kaneshige T, Owen RL and Jones AL : A modified method of fire-granular cationic iron colloid preparation : its use light and electron microscopic detection of anionic sites in the rat kidney glomerulus and certain other tissues. Arch Histol Jpn (1986) 49, 13-23.
2) 大塚愛二,宇野芳史,田口勇仁,村上宅郎:陽性および陰性荷電鉄コロイドによる組織・細胞荷電基の光顕ならびに電顕的検出.岡山医誌(1991)103,11-18.
3)Ohtsuka A and Murakami T : Fine anionic iron colloid and its use in light and electron microscopic detection of cationic sites in the rat kidney. Arch Histol Jpn (1986) 49, 543-552.
4) Fujita SC, Tada Y, Murakami F, Hayashi M and Matsumura M : Glycosaminoglycan-related epitopes surrounding different subsets of mammalian central neurons. Neurosci Res (1989) 7, 117-130.
5) 池田順一,川上速人,浅野 務,高田邦昭,平野 寛,平川公義:砂鼠海馬神経細胞の糖鎖構造について―レクチン法による組織化学的解析―.脳神経(1991)43,539-543.
6) Mulligan KA, Van Brederode JFM and Hendrickson AE : The lectin Vicia Villosa labels a distinct subset of GABAergic cells in macaque visual cortex. Visual Neurosci (1989) 2, 63-72.
7) Drake CT, Mulligan KA, Wimpey TL, Hendrickson A and Chavkin C : Characterization of Vicia villosa agglutinin-labeled GABAergic interneurons in the hippocampal formation and in acutely dissociated hippocampus. Brain Res (1991) 554, 176-185.
言語
Japanese
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma