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ID 30495
JaLCDOI
フルテキストURL
Thumnail fulltext.pdf 1.22 MB
著者
Tsubouchi, Yutaka Okayama University
Tsubouchi, Mari Okayama University
Hitomi, Sayoko Okayama Univeristy
Ohtsuka, Aiji Okayama University
Murakami, Takuro Okayama University
抄録

Neurons of cerebellar nuclei in the rat brain had a marked surface coat which was stained with cationic iron colloid or aldehyde fuchsin. Neurons with a similar surface coat were also noted in the retrosplenial cortex. The surface coat was stained doubly with cationic iron colloid and aldehyde fuchsin. Digestion with hyaluronidase eliminated the stainability of the surface coat to both agents. Combined digestion with chondroitinase ABC, heparitinase and keratanase eliminated the cationic iron colloid staining but did not interfere with the aldehyde fuchsin staining. Electron microscopy of ultrathin sections revealed that the iron particles were deposited in the perineuronal tissue spaces. These findings indicate that the surface coat consists of sulfated proteoglycans which occupy, as the extracellular matrix, the perineuronal tissue spaces. Many neurons in the retrosplenial cortex were labeled with lectin Vicia villosa agglutinin. Double staining revealed that these lectin-labeled neurons are usually reactive to cationic iron colloid. Few neurons in the cerebellar nuclei were labeled with lectin V. villosa agglutinin.

キーワード
perineuronal sulfated proteoglycans
cationic iron colloid staining
aldehyde fuchsin staining
lectin VVA labeling
rat brain
Amo Type
Article
発行日
1996-10
出版物タイトル
Acta Medica Okayama
50巻
5号
出版者
Okayama University Medical School
開始ページ
237
終了ページ
241
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT