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ID 32865
JaLCDOI
フルテキストURL
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著者
Murai, Hiroyasu Department of Occupational Therapy, School of Health Science, Kibi International University
Hiragami, Fukumi Department of Physical Therapy, School of Health Science, Kibi International University
Kawamura, Kenji Department of Physical Therapy, School of Health Science, Kibi International University
Motoda, Hirotoshi Department of Physical Therapy, School of Health Science, Kibi International University
Koike, Yoshihisa Department of Occupational Therapy, Faculty of Health and Welfare, Prefectural University of Hiroshima
Inoue, Shigeki Department of Cellular and Molecular Biology, Research Institute of Health and Welfare, Kibi International University
Kumagishi, Kanae Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Ohtsuka, Aiji Department of Human Morphology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Kano, Yoshio Department of Occupational Therapy, School of Health Science, Kibi International University
抄録

Among the 3 mitogen-activated protein kinases -- ERK, p38 MAPK and JNK -- JNK has been suggested to participate in apoptosis, whereas p38 MAPK is thought to be part of the differentiation response. There are many common inducers of JNK and p38 MAPK, but the mechanisms underlying the differential response to apoptosis and differentiation are poorly understood. We found that heatshock activated p38 MAPK at 3min after exposure to a temperature of 44 in stress-hypersensitive PC12m3 mutant cells, while it activated JNK at 20min after the same heat treatment. However, heat shock activated p38 MAPK 5min after heat treatment and JNK 10min after heat treatment in PC12 parental cells. The extent of phosphorylation of p38 MAPK induced by heat shock in PC12m3 cells was significantly greater than that in PC12 parental cells, and a high level of heat-shock-induced neurite outgrowth was observed only in PC12m3 cells. On the other hand, heat-shock-induced JNK activation appeared more quickly and apoptosis started earlier in PC12 parental cells. These findings indicate that short stress induces p38 MAPK and longer stress induces JNK, and that the response of these kinases to heat shock differs depending on cell type.

キーワード
heat shock
neurite outgrowth
p38 MAP kinase
JNK
PC12 mutant cells
Amo Type
Original Article
発行日
2010-02
出版物タイトル
Acta Medica Okayama
64巻
1号
出版者
Okayama University Medical School
開始ページ
55
終了ページ
62
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Sience KeyUT