著者 大塚 愛二|
発行日 2005-05-20
出版物タイトル 岡山医学会雑誌
117巻
1号
資料タイプ 学術雑誌論文
著者 村上 慎一郎| 松岡 宏明| 普山 康弘| 田口 勇仁| 大塚 愛二| 村上 宅郎|
発行日 2001-04-28
出版物タイトル 岡山医学会雑誌
113巻
1号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 蘇 衛東| 洪 珞珈| 朴 大勛| 大塚 愛二| 瀬尾 憲治|
発行日 1997-12-25
出版物タイトル 岡山医学会雑誌
109巻
7-12号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 人見 さよ子| 佐藤 徹| 朴 大勛| 大塚 愛二| 田口 勇仁|
発行日 1996-06-29
出版物タイトル 岡山医学会雑誌
108巻
3-6号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 大塚 愛二| 朴 大勛|
発行日 1995-10-31
出版物タイトル 岡山医学会雑誌
107巻
9-10号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 大塚 愛二|
発行日 1996-02-29
出版物タイトル 岡山医学会雑誌
107巻
11-12号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 朴 大勛| 大塚 愛二| 西田 圭一郎|
発行日 1996-10-31
出版物タイトル 岡山医学会雑誌
108巻
9-10号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 大塚 愛二| 山名 征三|
発行日 1996-10-31
出版物タイトル 岡山医学会雑誌
108巻
9-10号
資料タイプ 学術雑誌論文
著者 村上 宅郎| 田口 勇仁| 大塚 愛二|
発行日 1993-02-27
出版物タイトル 岡山医学会雑誌
105巻
1-2号
資料タイプ 学術雑誌論文
著者 宋 振嵐| 菊田 彰夫| 大谷 修| 大塚 愛二| 村上 宅郎| 佐能 正|
発行日 1988
出版物タイトル 岡山医学会雑誌
100巻
1-2号
資料タイプ 学術雑誌論文
著者 劉 晶潔| 大谷 修| 菊田 彰夫| 大塚 愛二| 田口 勇仁| 村上 宅郎| 佐能 正|
発行日 1988
出版物タイトル 岡山医学会雑誌
100巻
1-2号
資料タイプ 学術雑誌論文
JaLCDOI 10.18926/AMO/30373
フルテキストURL fulltext.pdf
著者 Okada, Satoko| Ohtsuka, Aiji| Akagi, Hirofumi| Nishizaki, Kazunori| Masuda, Yu|
抄録 <p>It has previously been confirmed that the guinea pig has aggregations of 10-20 lymphoid follicles at the junction of the nasal cavity and the nasopharyngeal duct. The vascular architecture of this nasal-associated lymphoid tissue (NALT) was studied by the corrosion cast/scanning electron microscope method. The NALT was supplied by branches of the inferior nasal artery. These afferent arterial branches gave off arterioles to the follicles and the interfollicular regions, where the arterioles ramified into capillaries. Some of these arterioles reached the subepithelial region to form a single-layer dense capillary network. The subepithelial capillaries gathered into short collecting venules, which in turn drained into high endothelial venules (HEV) in the interfollicular region. The HEV, which also receives tributaries from the follicular and interfollicular capillary plexuses, descended in the interfollicular regions and finally flowed into the efferent veins at the bottom of the NALT. Indentations impressed by high endothelial cells (HEC) were prominent on the surface of the HEV casts, and their frequency was larger in the upper course or segments than in the lower. This suggests that the incidence of HEC in the upper segments is higher than in the lower segments, and these findings are consistent with the hypothesis that some substances which are taken up into the subepithelial capillaries and transported to the venules induce differentiation and maintain of HEVs.</p>
キーワード nasal-associated lymphoid tisse vascular corrosion cast microvascular architecture high endothelial venule guinea pig
Amo Type Article
発行日 1995-08
出版物タイトル Acta Medica Okayama
49巻
4号
出版者 Okayama University Medical School
開始ページ 213
終了ページ 219
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 7502682
Web of Sience KeyUT A1995RR97800006
JaLCDOI 10.18926/AMO/30483
フルテキストURL fulltext.pdf
著者 Tsubouchi, Mari| Tsubochi, Yutaka| Hitomi, Sayoko| Ohtsuka, Aiji| Murakami, Takuro|
抄録 <p>Many neurons in the adult rat cingulate cortex possess perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectin Vicia villosa or soybean agglutinin. The perineuronal sulfated proteoglycans develop three to four weeks after birth. The cell surface glycoproteins develop at earlier stage or two to three weeks after birth. Dark or active neurons begin to appear three to four weeks after birth. These findings indicate that the brain matures after birth or during weaning period.</p>
キーワード rat brain perineuronal sulfated proteoglycans cell surface glycoproteins dark neurons
Amo Type Article
発行日 1996-12
出版物タイトル Acta Medica Okayama
50巻
6号
出版者 Okayama University Medical School
開始ページ 313
終了ページ 317
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8985468
Web of Sience KeyUT A1996WA04500005
JaLCDOI 10.18926/AMO/30495
フルテキストURL fulltext.pdf
著者 Tsubouchi, Yutaka| Tsubouchi, Mari| Hitomi, Sayoko| Ohtsuka, Aiji| Murakami, Takuro|
抄録 <p>Neurons of cerebellar nuclei in the rat brain had a marked surface coat which was stained with cationic iron colloid or aldehyde fuchsin. Neurons with a similar surface coat were also noted in the retrosplenial cortex. The surface coat was stained doubly with cationic iron colloid and aldehyde fuchsin. Digestion with hyaluronidase eliminated the stainability of the surface coat to both agents. Combined digestion with chondroitinase ABC, heparitinase and keratanase eliminated the cationic iron colloid staining but did not interfere with the aldehyde fuchsin staining. Electron microscopy of ultrathin sections revealed that the iron particles were deposited in the perineuronal tissue spaces. These findings indicate that the surface coat consists of sulfated proteoglycans which occupy, as the extracellular matrix, the perineuronal tissue spaces. Many neurons in the retrosplenial cortex were labeled with lectin Vicia villosa agglutinin. Double staining revealed that these lectin-labeled neurons are usually reactive to cationic iron colloid. Few neurons in the cerebellar nuclei were labeled with lectin V. villosa agglutinin.</p>
キーワード perineuronal sulfated proteoglycans cationic iron colloid staining aldehyde fuchsin staining lectin VVA labeling rat brain
Amo Type Article
発行日 1996-10
出版物タイトル Acta Medica Okayama
50巻
5号
出版者 Okayama University Medical School
開始ページ 237
終了ページ 241
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8914676
Web of Sience KeyUT A1996VQ20600002
JaLCDOI 10.18926/AMO/30497
フルテキストURL fulltext.pdf
著者 Tanaka, Toshihisa| Tsubouchi, Mari| Tsubouchi, Yutaka| Ohtsuka, Aiji| Murakami, Takuro|
抄録 <p>The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.</p>
キーワード parathyroid gland cationic and anionic iron colloid stainings vascular casting freeze fracture maceration
Amo Type Article
発行日 1996-10
出版物タイトル Acta Medica Okayama
50巻
5号
出版者 Okayama University Medical School
開始ページ 242
終了ページ 253
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 8914677
Web of Sience KeyUT A1996VQ20600003
JaLCDOI 10.18926/AMO/30753
フルテキストURL fulltext.pdf
著者 Nakatani, Satoru| Naito, Ichiro| Momota, Ryusuke| Hinenoya, Noriko| Horiuchi, Kanji| Nishida, Keiichiro| Ohtsuka, Aiji|
抄録 <p>We attempted to prepare colloidal iron within tissues by means of microwave irradiation. Mouse tissue blocks were fixed with a mixture of paraformaldehyde and ferric chloride in a cacodylate buffer, immersed in a cacodylate buffered ferric chloride solution, and irradiated in a microwave processor. Colloidal iron was prepared within tissues or cells, and was observed in the form of electron dense fine granules (1-2 nm in diameter) by transmission electron microscopy. Collagen fibrils in the connective tissue showed colloidal iron deposition at regular periodical intervals. Cells in the splenic tissue showed that fine colloidal granules were deposited on the ribosomes but not on the nuclear chromatin. This finding suggests that ferric ions could not diffuse into the nucleus, which was surrounded by the nuclear envelope. The podocyte processes of the renal glomerulus were stained diffusedly. Though this microwave in situ colloidal iron preparation method has some limitations, it is convenient for use in biomedical specimen preparation in transmission electron microscopy.</p>
キーワード colloidal iron microwave histochemistry transmission electron microscopy
Amo Type Article
発行日 2006-02
出版物タイトル Acta Medica Okayama
60巻
1号
出版者 Okayama University Medical School
開始ページ 59
終了ページ 64
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16508690
Web of Sience KeyUT 000235538900007
JaLCDOI 10.18926/AMO/30773
フルテキストURL fulltext.pdf
著者 Hitomi, Sayoko| Su, Wei Dong| Hong, Luo Jia| Ohtsuka, Aiji| Murakami, Takuro|
抄録 <p>Sections of the visual cortex of newborn (1-4 weeks after birth) and adult cats were stained with cationic iron colloid, aldehyde fuchsin or lectins (lectin Vicia villosa, soybean and Wisteria floribunda agglutinins). Many neurons in the adult cat visual cortex contained perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectins. Double staining indicated that some of the lectin-labeled neurons were not stained with cationic iron colloid, and also that some of the cationic iron colloid-stained neurons were not labeled with lectins. The perineuronal sulfated proteoglycans and cell surface glycoproteins developed 3 weeks after birth. In the newborn cats 1-2 weeks after birth, no neurons were reactive to cationic iron colloid, aldehyde fuchsin or lectins. In the newborn cats 34 weeks after birth, it was clearly observed that the cytoplasm of the glial cells closely associated with the neurons containing the perineuronal sulfated proteoglycans showed an intense reaction to cationic iron colloid and aldehyde fuchsin, and that the Golgi complexes of the neurons with cell surface glycoproteins were intensely labeled with lectins. These findings suggest that the perineuronal sulfated proteoglycans are derived from the associated glial cells, and that the cell surface glycoproteins are produced by the associated nerve cells.</p>
キーワード cat brain perineuronal sulfated proteoglycans cell surface glycoproteins cationic iron colloid aldehyde fuchsin lectin
Amo Type Article
発行日 1997-12
出版物タイトル Acta Medica Okayama
51巻
6号
出版者 Okayama University Medical School
開始ページ 295
終了ページ 299
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 9439770
Web of Sience KeyUT 000071183400001
JaLCDOI 10.18926/AMO/31000
フルテキストURL fulltext.pdf
著者 Yamamoto, Chugo| Murakami, Takuro| Ohtsuka, Aiji|
抄録 <p>The deep palmar muscles in monkey hands were studied. The contrahentes muscles mainly arose from the capitate bone, descended palmar to the deep palmar branch of the ulnar nerve and the palmar metacarpophalangeal nerves, and attached to the proximal phalanges or wing tendons of the second, fourth and fifth fingers. In relation to the deep palmar branch of the ulnar nerve and the palmar metacarpophalangeal nerves, the contrahentes muscles are homologous with the adductor pollicis and flexor indicis radialis muscles. The contrahentes muscles occasionally gave off some accessory slips which blended with the interosseous muscles. These findings suggest that the human adductor pollicis muscle is a well-developed remnant of a contrahens muscle, and that the human interosseous muscles contain some remnant of the contrahentes muscle. In fact, a well-developed remnant of a contrahens muscle was found in the fourth finger of a human hand. It is further considered that the human adductor pollicis muscle contains an element of the interosseous muscle of the thumb.</p>
キーワード monkey hands contrahentes muscles adductor pollicis muscle flexor indicis radialis musle interosseous muscles
Amo Type Article
発行日 1988-08
出版物タイトル Acta Medica Okayama
42巻
4号
出版者 Okayama University Medical School
開始ページ 215
終了ページ 226
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3177007
Web of Sience KeyUT A1988P884600005
JaLCDOI 10.18926/AMO/31005
フルテキストURL fulltext.pdf
著者 Ikebuchi, Yoshifumi| Murakami, Takuro| Ohtsuka, Aiji|
抄録 <p>The interosseous and lumbrical muscles in twenty-five hands of Japanese adult cadavers were dissected. The palmar and dorsal interosseous muscles continued, with few exceptions, into the wing tendons. The dorsal interosseous muscles gave off tendons which pierced the transverse laminae or passed deep to the transverse laminae, and attached to the bases of the proximal phalanges. The palmar interosseous muscles seldom had such attachments. The palmar and dorsal interosseous muscles sometimes gave off additional tendons which passed superficial to the transverse laminae and attached to the bases of the proximal phalanges. These latter attachments were typical in the contrahentes muscles. Thus, the present findings suggest that the human dorsal interosseous muscles are composite muscles derived from the dorsal abductor, flexor brevis and contrahens muscles, and that the human palmar interosseous muscles are composite muscles derived from the flexor brevis and contrahens muscles. The lumbrical muscles rarely gave off accessory slips with atavistic attachments to the proximal phalanges.</p>
キーワード human hand palmar interosseous muscles dorsal interosseous muslcles lumbrical muscles
Amo Type Article
発行日 1988-12
出版物タイトル Acta Medica Okayama
42巻
6号
出版者 Okayama University Medical School
開始ページ 327
終了ページ 334
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 3239437
Web of Sience KeyUT A1988R743300004