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ID 57898
フルテキストURL
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著者
Ozuru, Ryo Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University
Wakao, Shohei Department of Stem Cell Biology and Histology, Graduate School of Medicine, Tohoku University
Tsuji, Takahiro Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University
Ohara, Naoya Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Kaken ID researchmap
Matsuba, Takashi Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University
Amuran, Muhammad Y. Department of Neurology, Hasanuddin University Faculty of Medicine
Isobe, Junko Department of Bacteriology, Toyama Institute of Health
Iino, Morio Division of Legal Medicine, Department of Social Medicine, Faculty of Medicine, Tottori University
Nishida, Naoki Department of Legal Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
Matsumoto, Sari Department of Forensic Medicine, The Jikei University School of Medicine
Iwadate, Kimiharu Department of Forensic Medicine, The Jikei University School of Medicine
Konishi, Noriko Department of Food Microbiology, Tokyo Metropolitan Institute of Public
Yasuda, Kaori Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
Tashiro, Kosuke Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
Hida, Misato Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University
Yadoiwa, Arisato Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University
Kato, Shinsuke Division of Neuropathology, Department of Brain and Neuroscience, Faculty of Medicine, School of Medicine, Tottori University Faculty of Medicine
Yamashita, Eijiro Division of Clinical Radiology, Tottori University Hospital
Matsumoto, Sohkichi Department of Bacteriology, Niigata University School of Medicine
Kurozawa, Yoichi Division of Health Administration and Promotion, Department of Social Medicine, Faculty of Medicine, Tottori University
Dezawa, Mari Department of Stem Cell Biology and Histology, Graduate School of Medicine, Tohoku University
Fujii, Jun Division of Bacteriology, Department of Microbiology and Immunology, Faculty of Medicine, Tottori University
抄録
Shiga toxin-producing Escherichia coli (STEC) causes hemorrhagic colitis, hemolytic uremic syndrome, and acute encephalopathies that may lead to sudden death or severe neurologic sequelae. Current treatments, including immunoglobulin G (IgG) immunoadsorption, plasma exchange, steroid pulse therapy, and the monoclonal antibody eculizumab, have limited effects against the severe neurologic sequelae. Multilineage-differentiating stress-enduring (Muse) cells are endogenous reparative non-tumorigenic stem cells that naturally reside in the body and are currently under clinical trials for regenerative medicine. When administered intravenously, Musecells accumulate to the damaged tissue, where they exert anti-inflammatory, anti-apoptotic, anti-fibrotic, and immunomodulatory effects, and replace damaged cells by differentiating into tissue-constituent cells. Here, severely immunocompromised non-obese diabetic/severe combined immunodeficiency (NOD-SCID) mice orally inoculated with 9 × 109 colony-forming units of STEC O111 and treated 48 h later with intravenous injection of 5 × 104 Muse cells exhibited 100% survival and no severe after-effects of infection. Suppression of granulocyte-colony-stimulating factor (G-CSF) by RNAi abolished the beneficial effects of Muse cells, leading to a 40% death and significant body weight loss, suggesting the involvement of G-CSF in the beneficial effects of Muse cells in STEC-infected mice. Thus, intravenous administration of Muse cells could be a candidate therapeutic approach for preventing fatal encephalopathy after STEC infection.
キーワード
Muse cells
Shiga toxin-producing Escherichia coli
acute encephalopathy
発行日
2020-01-08
出版物タイトル
Molecular Therapy
28巻
1号
出版者
Elsevier
開始ページ
100
終了ページ
118
ISSN
15250016
NCID
AA11475060
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2019 The Author(s).
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1016/j.ymthe.2019.09.023
ライセンス
http://creativecommons.org/licenses/by/4.0/
Citation
Ryo Ozuru, Shohei Wakao, Takahiro Tsuji, Naoya Ohara, Takashi Matsuba, Muhammad Y. Amuran, Junko Isobe, Morio Iino, Naoki Nishida, Sari Matsumoto, Kimiharu Iwadate, Noriko Konishi, Kaori Yasuda, Kosuke Tashiro, Misato Hida, Arisato Yadoiwa, Shinsuke Kato, Eijiro Yamashita, Sohkichi Matsumoto, Yoichi Kurozawa, Mari Dezawa, Jun Fujii, Rescue from Stx2-Producing E. coli-Associated Encephalopathy by Intravenous Injection of Muse Cells in NOD-SCID Mice, Molecular Therapy, Volume 28, Issue 1, 2020, Pages 100-118, ISSN 1525-0016, https://doi.org/10.1016/j.ymthe.2019.09.023.
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