JaLCDOI 10.18926/AMO/53521
フルテキストURL 69_3_145.pdf
著者 Ishii, Hiroko| Kamikawa, Shigeshi| Hirohata, Satoshi| Mizutani, Akifumi| Abe, Koji| Seno, Masaharu| Oohashi, Toshitaka| Ninomiya, Yoshifumi|
抄録 Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis.
キーワード ECP reactive oxygen species Akt ERK
Amo Type Original Article
発行日 2015-06
出版物タイトル Acta Medica Okayama
69巻
3号
出版者 Okayama University Medical School
開始ページ 145
終了ページ 153
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 26101190
Web of Sience KeyUT 000356903000003
タイトル(別表記) Role of COLXV/XVⅢ gene, Multiplexin, as a basement membrane toolkit
フルテキストURL 127_103.pdf
著者 大橋 俊孝|
キーワード 基底膜 ツールキット XV/XVⅢ型コラーゲン プロテオグリカン ミトコンドリア
備考 総説(Reviews)
出版物タイトル 岡山医学会雑誌
発行日 2015-08-03
127巻
2号
出版者 岡山医学会
出版者(別表記) Okayama Medical Association
開始ページ 103
終了ページ 109
ISSN 0030-1558
NCID AN00032489
資料タイプ 学術雑誌論文
関連URL isVersionOf https://doi.org/10.4044/joma.127.103
言語 Japanese
著作権者 Copyright (c) 2015 岡山医学会
論文のバージョン publisher
査読 有り
DOI 10.4044/joma.127.103
NAID 130005096251
Sort Key 5
Eprints Journal Name joma
参考文献 1) Hynes RO : The evolution of metazoan extracellular matrix. J Cell Biol (2012) 196, 671-679. 2) Yurchenco PD : Basement membranes : cell scaffoldings and signaling platforms. Cold Spring Harb Perspect Biol (2011) 3, pii : a004911. 3) Hutter H, Vogel BE, Plenefisch JD, Norris CR, Proenca RB, Spieth J, Guo C, Mastwal S, Zhu X, Scheel J, Hedgecock EM : Conservation and novelty in the evolution of cell adhesion and extracellular matrix genes. Science (2000) 287, 989-994. 4) Hynes RO, Zhao Q : The evolution of cell adhesion. J Cell Biol (2000) 150, F89-96. 5) Ackley BD, Crew JR, Elamaa H, Pihlajaniemi T, Kuo CJ, Kramer JM : The NC1/endostatin domain of Caenorhabditis elegans type XVIII collagen affects cell migration and axon guidance. J Cell Biol (2001) 152, 1219-1232. 6) Meyer and Moussian : Drosophila multiplexin (Dmp) modulates motor axon pathfinding accuracy. Dev Growth Differ (2009) 51, 483-498. 7) Momota R, Naito I, Ninomiya Y, Ohtsuka A : Drosophila type XV/XVIII collagen, Mp, is involved in Wingless distribution. Matrix Biol (2011) 30, 258-266. 8) Whittaker CA, Bergeron KF, Whittle J, Brandhorst BP, Burke RD, Hynes RO : The echinoderm adhesome. Dev Biol (2006) 300, 252-266. 9) Abe N, Muragaki Y, Yoshioka H, Inoue H, Ninomiya Y : Identification of a novel collagen chain represented by extensive interruptions in the triple-helical region. Biochem Biophys Res Commun (1993) 196, 576-582. 10) Oh SP, Kamagata Y, Muragaki Y, Timmons S, Ooshima A, Olsen BR : Isolation and sequencing of cDNAs for proteins with multiple domains of Gly-Xaa-Yaa repeats identify a distinct family of collagenous proteins. Proc Natl Acad Sci U S A (1994) 91, 4229-4233. 11) Muragaki Y, Abe N, Ninomiya Y, Olsen BR, Ooshima A : The human alpha 1 (XV) collagen chain contains a large amino-terminal non-triple helical domain with a tandem repeat structure and homology to alpha 1 (XVIII) collagen. J Biol Chem (1994) 269, 4042-4046. 12) Rehn M, Pihlajaniemi T : Alpha 1 (XVIII), a collagen chain with frequent interruptions in the collagenous sequence, a distinct tissue distribution, and homology with type XV collagen. Proc Natl Acad Sci U S A (1994) 91, 4234-4238. 13) Tomono Y, Naito I, Ando K, Yonezawa T, Sado Y, Hirakawa S, Arata J, Okigaki T, Ninomiya Y : Epitope-defined monoclonal antibodies against multiplexin collagens demonstrate that type XV and XVIII collagens are expressed in specialized basement membranes. Cell Struct Funct (2002) 27, 9-20. 14) Muragaki Y, Timmons S, Griffith CM, Oh SP, Fadel B, Quertermous T, Olsen BR : Mouse Col18a1 is expressed in a tissue-specific manner as three alternative variants and is localized in basement membrane zones. Proc Natl Acad Sci U S A (1995) 92, 8763-8767. 15) Rehn M, Pihlajaniemi T : Identification of three N-terminal ends of type XVIII collagen chains and tissue-specific differences in the expression of the corresponding transcripts. The longest form contains a novel motif homologous to rat and Drosophila frizzled proteins. J Biol Chem (1995) 270, 4705-4711. 16) Fukai N, Eklund L, Marneros AG, Oh SP, Keene DR, Tamarkin L, Niemelä M, Ilves M, Li E, Pihlajaniemi T, Olsen BR : Lack of collagen XVIII/endostatin results in eye abnormalities. EMBO J (2002) 21, 1535-1544. 17) Halfter W, Dong S, Schurer B, Cole GJ : Collagen XVIII is a basement membrane heparan sulfate proteoglycan. J Biol Chem (1998) 273, 25404-25412. 18) Li D, Clark CC, Myers JC : Basement membrane zone type XV collagen is a disulfide-bonded chondroitin sulfate proteoglycan in human tissues and cultured cells. J Biol Chem (2000) 275, 22339-22347. 19) Seppinen L, Pihlajaniemi T : The multiple functions of collagen XVIII in development and disease. Matrix Biol (2011) 30, 83-92. 20) Liétard J, Théret N, Rehn M, Musso O, Dargère D, Pihlajaniemi T, Clément B : The promoter of the long variant of collagen XVIII, the precursor of endostatin, contains liver-specific regulatory elements. Hepatology (2000) 32, 1377-1385. 21) Saarela J, Rehn M, Oikarinen A, Autio-Harmainen H, Pihlajaniemi T : The short and long forms of type XVIII collagen show clear tissue specificities in their expression and location in basement membrane zones in humans. Am J Pathol (1998) 153, 611-626. 22) Ylikärppä R, Eklund L, Sormunen R, Kontiola AI, Utriainen A, Määttä M, Fukai N, Olsen BR, Pihlajaniemi T : Lack of type XVIII collagen results in anterior ocular defects. FASEB J (2003) 17, 2257-2259. 23) Määttä M, Heljasvaara R, Pihlajaniemi T, Uusitalo M : Collagen XVIII/endostatin shows a ubiquitous distribution in human ocular tissues and endostatin-containing fragments accumulate in ocular fluid samples. Graefes Arch Clin Exp Ophthalmol (2007) 245, 74-81. 24) O'Reilly MS, Boehm T, Shing Y, Fukai N, Vasios G, Lane WS, Flynn E, Birkhead JR, Olsen BR, Folkman J : Endostatin : an endogenous inhibitor of angiogenesis and tumor growth. Cell (1997) 88, 277-285. 25) Hohenester E, Sasaki T, Olsen BR, Timpl R : Crystal structure of the angiogenesis inhibitor endostatin at 1.5 A resolution. EMBO J (1998) 17, 1656-1664. 26) Sasaki T, Larsson H, Kreuger J, Salmivirta M, Claesson-Welsh L, Lindahl U, Hohenester E, Timpl R : Structural basis and potential role of heparin/heparan sulfate binding to the angiogenesis inhibitor endostatin. EMBO J (1999) 18, 6240-6248. 27) Iozzo RV, Schaefer L : Proteoglycan form and function : A comprehends nomenclature of proteoglycans. Matrix Biol (2015) in press. 28) Marneros AG, Olsen BR : Physiological role of collagen XVIII and endostatin. FASEB J (2005) 19, 716-728. 29) Mongiat M, Sweeney SM, San Antonio JD, Fu J, Iozzo RV : Endorepellin, a novel inhibitor of angiogenesis derived from the C terminus of perlecan. J Biol Chem (2003) 278, 4238-4249. 30) Sasaki T, Fukai N, Mann K, Göhring W, Olsen BR, Timpl R : Structure, function and tissue forms of the C-terminal globular domain of collagen XVIII containing the angiogenesis inhibitor endostatin. EMBO J (1998) 17, 4249-4256. 31) Sertié AL, Sossi V, Camargo AA, Zatz M, Brahe C, Passos-Bueno MR : Collagen XVIII, containing an endogenous inhibitor of angiogenesis and tumor growth, plays a critical role in the maintenance of retinal structure and in neural tube closure (Knobloch syndrome). Hum Mol Genet (2000) 9, 2051-2058. 32) Suzuki OT, Sertié AL, Der Kaloustian VM, Kok F, Carpenter M, Murray J, Czeizel AE, Kliemann SE, Rosemberg S, Monteiro M, Olsen BR, Passos-Bueno MR : Molecular analysis of collagen XVIII reveals novel mutations, presence of a third isoform, and possible genetic heterogeneity in Knobloch syndrome. Am J Hum Genet (2002) 71, 1320-1329. 33) Fukai N, Eklund L, Marneros AG, Oh SP, Keene DR, Tamarkin L, Niemelä M, Ilves M, Li E, Pihlajaniemi T, Olsen BR : Lack of collagen XVIII/endostatin results in eye abnormalities. EMBO J (2002) 21, 1535-1544. 34) Ylikärppä R, Eklund L, Sormunen R, Kontiola AI, Utriainen A, Määttä M, Fukai N, Olsen BR, Pihlajaniemi T : Lack of type XVIII collagen results in anterior ocular defects. FASEB J (2003) 17, 2257-2259. 35) Moulton KS, Olsen BR, Sonn S, Fukai N, Zurakowski D, Zeng X : Loss of collagen XVIII enhances neovascularization and vascular permeability in atherosclerosis. Circulation (2004) 110, 1330-1336. 36) Kinnunen AI, Sormunen R, Elamaa H, Seppinen L, Miller RT, Ninomiya Y, Janmey PA, Pihlajaniemi T : Lack of collagen XVIII long isoforms affects kidney podocytes, whereas the short form is needed in the proximal tubular basement membrane. J Biol Chem (2011) 286, 7755-7764. 37) Zaferani A, Talsma DT, Yazdani S, Celie JW, Aikio M, Heljasvaara R, Navis GJ, Pihlajaniemi T, van den Born J : Basement membrane zone collagens XV and XVIII/proteoglycans mediate leukocyte influx in renal ischemia/reperfusion. PLoS One (2014) 9, e106732. 38) Eklund L, Piuhola J, Komulainen J, Sormunen R, Ongvarrasopone C, Fässler R, Muona A, Ilves M, Ruskoaho H, Takala TE, Pihlajaniemi T : Lack of type XV collagen causes a skeletal myopathy and cardiovascular defects in mice. Proc Natl Acad Sci U S A (2001) 98, 1194-1199. 39) Ackley BD, Kang SH, Crew JR, Suh C, Jin Y, Kramer JM : The basement membrane components nidogen and type XVIII collagen regulate organization of neuromuscular junctions in Caenorhabditis elegans. J Neurosci (2003) 23, 3577-3587. 40) Rasi K, Piuhola J, Czabanka M, Sormunen R, Ilves M, Leskinen H, Rysä J, Kerkelä R, Janmey P, Heljasvaara R, Peuhkurinen K, Vuolteenaho O, et al. : Collagen XV is necessary for modeling of the extracellular matrix and its deficiency predisposes to cardiomyopathy. Circ Res (2010) 107, 1241-1252. 41) Momota R, Narasaki M, Komiyama T, Naito I, Ninomiya Y, Ohtsuka A : Drosophila type XV/XVIII collagen mutants manifest integrin mediated mitochondrial dysfunction, which is improved by cyclosporin A and losartan. Int J Biochem Cell Biol (2013) 45, 1003-1011.