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ID 52119
フルテキストURL
著者
Muraoka, Takayuki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Soh, Junichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg ORCID Kaken ID researchmap
Toyooka, Shinichi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac SurgPathol
Aoe, Keisuke Yamaguchi Ube Med Ctr, Natl Hosp Org, Dept Med Oncol & Clin Res
Fujimoto, Nobukazu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Canc & Thorac Surg
Hashida, Shinsuke Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Maki, Yuho Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Tanaka, Norimitsu Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Shien, Kazuhiko Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg ORCID Kaken ID researchmap
Furukawa, Masashi Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Yamamoto, Hiromasa Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg ORCID Kaken ID researchmap
Asano, Hiroaki Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg
Tsukuda, Kazunori Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg ORCID Kaken ID publons researchmap
Kishimoto, Takumi Okayama Rosai Hosp, Dept Resp Med
Otsuki, Takemi Kawasaki Med Univ, Dept Hyg
Miyoshi, Shinichiro Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Thorac Surg Kaken ID publons researchmap
抄録
Objectives: Malignant pleural mesothelioma (MPM) is an aggressive tumor with a poor prognosis. microRNA-34b/c (miR-34b/c), which plays an important role in the pathogenesis of MPM, is frequently downregulated by DNA methylation in approximately 90% of MPM cases. In this study, we estimated the degree of miR-34b/c methylation in serum-circulating DNA using a digital methylation specific PCR assay (MSP). Materials and methods: A real-time MSP assay was performed using the SYBR Green method. The melting temperature (Tm) of each PCR product was examined using a melting curve analysis. For a digital MSP assay, 40 wells were analyzed per sample. A total of 110 serum samples from 48 MPM cases, 21 benign asbestos pleurisy (BAP) cases, and 41 healthy volunteers (HVs) were examined. Results: Positive range of Tm value for miR-34b/c methylation was defined as 77.71-78.79 degrees C which was the mean 3 standard deviations of 40 wells of a positive control. The number of miR-34b/c methylated wells was counted per sample according to this criterion. The number of miR-34b/c methylated wells in MPM cases was significantly higher than that in BAP cases (P = 0.03) or HVs (P < 0.001). Advanced MPM cases tended to have higher number of miR-34b/c methylated wells than early MPM cases. Receiver-operating characteristic (ROC) curve analysis revealed that three number of miR-34b/c methylated wells per sample was the best cut-off of positivity of MPM with a 67% of sensitivity and a 77% specificity for prediction. The area under the ROC curve was 0.77. Conclusions: Our digital MSP assay can quantify miR-34b/c methylation in serum-circulating DNA. The degree of miR-34b/c methylation in serum-circulating DNA is associated with MPM, suggesting that this approach might be useful for the establishment of a new detection system for MPM.
キーワード
Digital PCR
Malignant pleural mesothelioma
microRNA
miR-34b/c
Methylation
Circulating DNA
発行日
2013-12
出版物タイトル
Lung Cancer
82巻
3号
出版者
Elsevier Ireland Ltd.
開始ページ
485
終了ページ
490
ISSN
0169-5002
NCID
AA10785743
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.1016/j.lungcan.2013.09.017
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/52509
言語
English
著作権者
(C) 2013 Elsevier Ireland Ltd. All rights reserved.
論文のバージョン
author
査読
有り
DOI
Web of Science KeyUT