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ID 31612
JaLCDOI
フルテキストURL
著者
Funamori, Yuka Okayama University
Fujinaga, Yukako Okayama University
Yokota, Kenji Okayama University
Inoue, Kaoru Okayama University
Hirai, Yoshikazu Jichi Medical School, Tochigi
Oguma, Keiji Okayama University Kaken ID researchmap
Kira, Shohei Okayama University
Taketa, Kazuhisa Okayama University
抄録

Three outbreaks and many isolated cases of enterohemorrhagic Escherichia coli O157:H7 occurred in 1996 and 1997 in Okayama Prefecture, Japan. In an attempt to investigate the route of these infections, the strains isolated from the 3 outbreaks (total 33 strains) and 15 isolated cases (total 15 strains) were investigated using random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE). In addition, 10 strains from an outbreak in Tojo Cho, Hiroshima Prefecture (June 1996), 2 strains from the particular types of meat in Kochi Prefecture, and 42 strains isolated from bovine feces in a farm in Okayama Prefecture were also investigated in the same manner. PFGE was much more useful than RAPD for molecular typing of the clinical isolates, in that it allowed us to classify them into 10 PFGE groups. We noted that the strains differed according to the time and place of the outbreaks (or isolated cases). This indicates that O157:H7 infections in Okayama Prefecture were caused by different strains (although some cases were aggravated by the same strains as were found in other areas). The isolates from bovine feces were classified into 5 groups by PFGE profiles, but none of them were identical to those of the clinical isolates.

キーワード
molecular epidemiology
enterohemorrhagic Escherichia coli O157: H7
pulsed field gel electrophoresis
random amplification of polymorphic DNA
Amo Type
Article
発行日
1999-08
出版物タイトル
Acta Medica Okayama
53巻
4号
出版者
Okayama University Medical School
開始ページ
193
終了ページ
200
ISSN
0386-300X
NCID
AA00508441
資料タイプ
学術雑誌論文
言語
English
論文のバージョン
publisher
査読
有り
PubMed ID
Web of Science KeyUT