フルテキストURL fulltext.pdf
著者 Tomiyama, Yasuaki| Shinohara, Tsugumichi| Matsuka, Mirai| Bando, Tetsuya| Mito, Taro| Tomioka, Kenji|
キーワード Circadian clock Clockwork orange Clock gene Cricket cry2 Molecular oscillation Locomotor rhythm
発行日 2020-11-11
出版物タイトル Zoological Letters
6巻
1号
出版者 BMC
開始ページ 12
ISSN 2056-306X
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © The Author(s). 2020
論文のバージョン publisher
DOI 10.1186/s40851-020-00166-4
Web of Science KeyUT 000588683700001
関連URL isVersionOf https://doi.org/10.1186/s40851-020-00166-4
フルテキストURL fulltext.pdf
著者 Habuta, Munenori| Yasue, Akihiro| Suzuki, Ken-Ichi T.| Fujita, Hirofumi| Sato, Keita| Kono, Hitomi| Takayama, Ayuko| Bando, Tetsuya| Miyaishi, Satoru| Oyadomari, Seiichi| Tanaka, Eiji| Ohuchi, Hideyo|
発行日 2020-10-15
出版物タイトル PLoS ONE
15巻
10号
出版者 Public Library of Science
開始ページ e0240333
ISSN 1932-6203
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 © 2020 Habuta et al.
論文のバージョン publisher
PubMed ID 33057360
DOI 10.1371/journal.pone.0240333
Web of Science KeyUT 000581814700082
関連URL isVersionOf https://doi.org/10.1371/journal.pone.0240333
JaLCDOI 10.18926/AMO/59950
フルテキストURL 74_3_199.pdf
著者 Fujita, Hirofumi| Bando, Tetsuya| Oyadomari, Seiichi| Ochiai, Kazuhiko| Watanabe, Masami| Kumon, Hiromi| Ohuchi, Hideyo|
抄録 Dickkopf 3 (Dkk3) is a secreted protein belonging to the Dkk family and encoded by the orthologous gene of REIC. Dkk3/REIC is expressed by mouse and human adrenal glands, but the understanding of its roles in this organ is still limited. To determine the functions of Dkk3 in the mouse adrenal gland, we first identified that the mouse Dkk3 protein is N-glycosylated in the adrenal gland as well as in the brain. We performed proteome analysis on adrenal glands from Dkk3-null mice, in which exons 5 and 6 of the Dkk3 gene are deleted. Twodimensional polyacrylamide gel electrophoresis of adrenal proteins from wild-type and Dkk3-null mice revealed 5 protein spots whose intensities were altered between the 2 genotypes. Mass spectrometry analysis of these spots identified binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) chaperone. To determine whether mouse Dkk3 is involved in the unfolded protein response (UPR), we carried out a reporter assay using ER-stress responsive elements. Forced expression of Dkk3 resulted in the induction of distinct levels of reporter expression, showing the UPR initiated by the ER membrane proteins of activating transcription factor 6 (ATF6) and inositol-requring enzyme 1 (IRE1). Thus, it is possible that Dkk3 is a physiological ER stressor in the mouse adrenal gland.
キーワード Dkk3 knockout mouse adrenal gland glucose-regulated protein 78 proteome endoplasmic reticulum stress
Amo Type Original Article
発行日 2020-06
出版物タイトル Acta Medica Okayama
74巻
3号
出版者 Okayama University Medical School
開始ページ 199
終了ページ 208
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
著作権者 CopyrightⒸ 2020 by Okayama University Medical School
論文のバージョン publisher
査読 有り
PubMed ID 32577017
Web of Science KeyUT 000543363400003
NAID 120006862792
著者 Hamada, Yoshimasa| Bando, Tetsuya| Nakamura, Taro| Ishimaru, Yoshiyasu| Mito, Taro| Noji, Sumihare| Tomioka, Kenji| Ohuchi, Hideyo|
発行日 2015-09
出版物タイトル Development
142巻
17号
資料タイプ 学術雑誌論文
著者 板東 哲哉|
発行日 2015-10
出版物タイトル Okayama University Medical Research Updates
16巻
資料タイプ その他