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ID 61252
フルテキストURL
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著者
Nakano, Ryohei Experimental Farm of Graduate School of Agriculture, Kyoto University Kaken ID publons researchmap
Kawai, Takashi Graduate School of Environmental and Life Science, Okayama University
Fukamatsu, Yosuke Graduate School of Environmental and Life Science, Okayama University
Akita, Kagari Graduate School of Environmental and Life Science, Okayama University
Watanabe, Sakine Graduate School of Environmental and Life Science, Okayama University
Asano, Takahiro Graduate School of Environmental and Life Science, Okayama University
Takata, Daisuke Faculty of Food and Agricultural Sciences, Fukushima University
Sato, Mamoru Faculty of Food and Agricultural Sciences, Fukushima University
Fukuda, Fumio Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
Ushijima, Koichiro Graduate School of Environmental and Life Science, Okayama University ORCID Kaken ID publons researchmap
抄録
The postharvest properties of two ultra-late maturing peach cultivars, "Tobihaku" (TH) and "Daijumitsuto" (DJ), were investigated. Fruit were harvested at commercial maturity and held at 25 degrees C. TH exhibited the characteristics of normal melting flesh (MF) peach, including rapid fruit softening associated with appropriate level of endogenous ethylene production In contrast, DJ did not soften at all during 3 weeks experimental period even though considerable ethylene production was observed. Fruit of TH and DJ were treated with 5,000 ppm of propylene, an ethylene analog, continuously for 7 days. TH softened rapidly whereas DJ maintained high flesh firmness in spite of an increase in endogenous ethylene production, suggesting that DJ but not TH lacked the ability to be softened in response to endogenous and exogenous ethylene/propylene. DNA-seq analysis showed that tandem endo-polygalacturonase (endoPG) genes located at melting flesh (M) locus, Pp-endoPGM (PGM), and Pp-endoPGF (PGF), were deleted in DJ. The endoPG genes at M locus are known to control flesh texture of peach fruit, and it was suggested that the non-softening property of DJ is due to the lack of endoPG genes. On the other hand, TH possessed an unidentified M haplotype that is involved in determination of MF phenotype. Structural identification of the unknown M haplotype, designated as M-0, through comparison with previously reported M haplotypes revealed distinct differences between PGM on M-0 haplotype (PGM-M-0) and PGM on other haplotypes (PGM-M-1). Peach M haplotypes were classified into four main haplotypes: M-0 with PGM-M-0; M-1 with both PGM-M-1 and PGF; M-2 with PGM-M-1; and M-3 lacking both PGM and PGF. Re-evaluation of M locus in association with MF/non-melting flesh (NMF) phenotypes in more than 400 accessions by using whole genome shotgun sequencing data on database and/or by PCR genotyping demonstrated that M-0 haplotype was the common haplotype in MF accessions, and M-0 and M-1 haplotypes were dominant over M-2 and M-3 haplotypes and co-dominantly determined the MF trait. It was also assumed on the basis of structural comparison of M haplotypes among Prunus species that the ancestral haplotype of M-0 diverged from those of the other haplotypes before the speciation of Prunus persica.
キーワード
fruit
softening
ethylene
Prunus persica
melting flesh locus
endoPG
postharvest
発行日
2020-11-26
出版物タイトル
Frontiers in Plant Science
11巻
出版者
Frontiers Media
開始ページ
554158
ISSN
1664-462X
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
著作権者
© 2020 Nakano, Kawai, Fukamatsu, Akita, Watanabe, Asano, Takata, Sato, Fukuda and Ushijima.
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.3389/fpls.2020.554158
ライセンス
https://creativecommons.org/licenses/by/4.0/
助成機関名
文部科学省
助成番号
18H02200