JaLCDOI 10.18926/47025
フルテキストURL mfe_36_2_051_060.pdf
著者 Hikida, Masaki| Magari, Masaki| Nakayama, Yasunori| Kanayama, Naoki| Ohmori, Hitoshi|
抄録 A population of peripheral B cells have been shown to express recombination activating gene products, RAG-1 and RAG-2, which are considered to be involved in revising the B cell antigen receptor (BCR) in the periphery. BCR engagement has been reported to turn off RAG expression in peripheral B cells, whereas the same treatment has an opposite effect in immature B cells in the bone marrow. In contrast to receptor editing that is involved in the removal of autoreactivity in immature B cells, it has been shown that secondary V(D)J rearrangement in peripheral B cells, termed receptor revision, contributes to affinity maturation of antibodies. Here, we show that RAG-2 expression in murine splenic B cells was abrogated by the coligation of BCR with complement receptors (CD21/CD35) much more efficiently than by the engagement of BCR alone. On the other hand, the same coligation augmented proliferation of anti-CD40-stimulated B cells. Consistent with these observations, RAG-2 expression was lower in the draining lymph nodes of the quasi-monoclonal mice when they were immunized with a high-affinity antigen than with a low-affinity one. These findings suggest a crucial role for CD21/CD35 in directing the conservation or the revision of BCRs in peripheral B cells.
出版物タイトル Memoirs of the Faculty of Engineering, Okayama University
発行日 2002-03
開始ページ 51
終了ページ 60
ISSN 0475-0071
言語 English
論文のバージョン publisher
NAID 80015582223
JaLCDOI 10.18926/46955
フルテキストURL mfe_38_1-2_091_096.pdf
著者 Kanayama, Naoki| Yamakoshi, Kimi| Kiyomi, Masaaki| Magari, Masaki| Ohmori, Hitoshi|
抄録 Generally, IgM antibodies (Abs) produced in a primary immune response show lower affinity for an inducing antigen (Ag) compared with the corresponding IgG Abs that are major switched isotypes formed in the secondary response. An IgM molecule is a pentamer with 10 Ag-binding sites that will contribute to an increase of avidity for an Ag. To estimate the contribution of the pentameric structure to the avidity of an IgM Ab, we generated IgM and IgG1 monoclonal Abs (mAbs) with identical V regions that are specific for 4-hydroxy-3-nitrophenylacetyl (NP) by in vitro class switching of B cells followed by the cell fusion with a mouse myeloma cell line. Compared with an anti-NP IgG1 mAb, the corresponding IgM mAb showed much higher avidity for NP-conjugated bovine serum albumin, which was drastically reduced after being dissociated into monomers.
出版物タイトル Memoirs of the Faculty of Engineering, Okayama University
発行日 2004-03
開始ページ 91
終了ページ 96
ISSN 0475-0071
言語 English
論文のバージョン publisher
NAID 80017001822
著者 曲 正樹|
発行日 2002-09-30
資料タイプ 学位論文