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ID 33006
フルテキストURL
著者
Nakayasu, Hiroshi Department of Biology, Faculty of Science, Okayama University
Arata, Noriko Department of Biology, Faculty of Science, Okayama University
抄録
We produced a monoclonal antibody, named A20, which specifically recognizes a 35 kDa protein and stains myelinated axons in zebrafish brain. The A20 antigen is located at the outside of the myelin layer of large axons, and comprises a fine meshwork composed of thin unit fibers about 1–2 μm in length and about 100–200 nm in thickness. The unit fibers form pentagonal and hexagonal structures, which further polymerize into an envelope structure on the axons. The A20 monoclonal antibody did not stain neuronal cell bodies nor synapses. Instead, the distribution of the A20 antigen was along axons, practically coincident with the distribution of myelin basic protein. The monoclonal antibody stained only axons in the central nervous system (CNS), and not the extracellular matrix surrounding Schwann cells. These results suggest that this antigenic meshwork (which we call the periaxonal net) is synthesized by oligodendrocytes. During the development of the zebrafish brain, the periaxonal net appeared after the formation of myelin on the axons. The periaxonal net developed first at the brain stem, then gradually appeared at the caudal end of the spinal cord. The thickness of the periaxonal net around the Mauthner axon changed during development. Although the thickness of the Mauthner axon continues to grow throughout life, the thickness of periaxonal net stopped growing at 6 months after fertilization.
キーワード
Oligodendrocyte
Monoclonal antibody
Extracellular matrix
Central nervous system
備考
Digital Object Identifer:10.1016/S0006-8993(02)03888-X
Published with permission from the copyright holder. This is the author's copy, as published in Brain Research, 31 January 2003, Volume 961, Issue 2, Pages 179-189.
Publisher URL:http://dx.doi.org/10.1016/S0006-8993(02)03888-X
Direct access to Thomson Web of Science record
Copyright © 2002 Elsevier Science B.V. Limited. All rights reserved.
発行日
2003-01-31
出版物タイトル
Brain Research
961巻
2号
出版者
Elsevier Science B.V.
開始ページ
179
終了ページ
189
ISSN
0006-8993
NCID
AA0057324X
資料タイプ
学術雑誌論文
言語
English
著作権者
Elsevier Science B.V.
論文のバージョン
author
査読
有り
DOI
PubMed ID
Web of Science KeyUT
Submission Path
biology_general/19