JaLCDOI 10.18926/AMO/30749
フルテキストURL fulltext.pdf
著者 Komiyama, Takamitsu| Nishida, Keiichiro| Yorimitsu, Masanori| Doi, Hideyuki| Miyazawa, Shinichi| Kitamura, Ai| Yoshida, Aki| Nasu, Yoshihisa| Abe, Nobuhiro| Ozaki, Toshifumi|
抄録 Ossification disturbance in femoral head reportedly is seen in the Spontaneously Hypertensive rats (SHR) between ages of 10 and 20 weeks. We investigated serum and tissue levels of insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) in SHR relevant to the ossification disturbance and osteonecrosis of the femoral head. Serum levels of IGF-1 and VEGF were significantly lower in SHR than in Wistar Kyoto rats (WKY) at weeks 5, 10, 15 and 20 (p<0.005). The incidence of histological ossification disturbance of the femoral head was higher in SHR (59%) than in WKY (40%) at week 20. Lower serum and local levels of VEGF in SHR appeared to be related to the incomplete ossification of the femoral heads. Immunohistochemical study showed significantly lower numbers of IGF-1 and VEGF positive chondrocytes in the femoral epiphyseal cartilage of SHR than in those of WKY at weeks 10, 15 and 20. Our results suggest that local and/or systemic levels of IGF-1 and VEGF between ages of 5 and 20 weeks might play roles in the pathogenesis of ossifi cation disturbance of the femoral head in SHR.
キーワード spontaneous hypertensive rats insulin like growth factor-1 vascular endothelial growth factor ossification disturbance osteonecrosis
Amo Type Article
発行日 2006-06
出版物タイトル Acta Medica Okayama
60巻
3号
出版者 Okayama University Medical School
開始ページ 141
終了ページ 148
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 16838042
Web of Science KeyUT 000238503600001
JaLCDOI 10.18926/AMO/30739
フルテキストURL fulltext.pdf
著者 Aiga, Ayako| Asaumi, Koji| Lee, You Jin| Kadota, Hiroaki| Mitani, Shigeru| Ozaki, Toshifumi| Takigawa, Masaharu|
抄録 The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosinrelated kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis.
キーワード neurotrophin Trk distraction osteogenesis mechanical stress
Amo Type Article
発行日 2006-10
出版物タイトル Acta Medica Okayama
60巻
5号
出版者 Okayama University Medical School
開始ページ 267
終了ページ 277
ISSN 0386-300X
NCID AA00508441
資料タイプ 学術雑誌論文
言語 English
論文のバージョン publisher
査読 有り
PubMed ID 17072373
Web of Science KeyUT 000241509000003
著者 尾﨑 敏文|
発行日 2006-01-04
出版物タイトル 岡山医学会雑誌
117巻
3号
資料タイプ 学術雑誌論文
著者 国定 俊之| 尾﨑 敏文|
発行日 2008-05-01
出版物タイトル 岡山医学会雑誌
120巻
1号
資料タイプ 学術雑誌論文
フルテキストURL K000901.pdf
著者 尾崎 敏文|
発行日 1991-03-28
資料タイプ 学位論文
学位授与番号 甲第901号
学位授与年月日 1991-03-28
学位・専攻分野 博士(医学)
授与大学 岡山大学
言語 Japanese