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ID 10600
Eprint ID
10600
フルテキストURL
タイトル(別表記)
Molecular Mechanism of the Self-incompatibility in the Rosaceous Species
著者
牛島 幸一郎 岡山大学 ORCID Kaken ID publons researchmap
抄録
Self-incompatibility(GSI) in the rosaceous species is controlled by the S locus consisting of S-RNase gene and an unidentified 'pollen S' gene. A~200kbp of cosmid contig for the Sc haplotype of almond was constructed. Genomic Southern blot analyses showed that most cosmid end probes, except those near the Sc-RNase gene, cross-hybridized with DNA fragments from different S haplotypes, implying that the cosmid contig extends to the borders of the S locus. A~70kbp segment of the Sc haplotype, the S haplotype-specific region containing the S-RNase gene, was complentely sequenced. This regin was found to contain two pollen-expressed F-box genes that are likely candidates for pollen S genes. One of them, named SFB(S haplotype-specific F-Box protein), was specifically expressed in pollen, and showed high level of S haplotype-specific sequence polymorphism, comparable to that of the S-RNases. The other is unlikely to determine the S specificity of pollen, because it showed little allelic sequence polymorphism and was expressed also in pistil. Three other S haplotypes were cloned and the pollen-expressed genes were physically mapped. In all four cases, SFBs were physically linked to the S-RNase genes, and were located at the S haplotype-specific region, suggesting that the two genes are inherited as aunit. These features are consistent with the hypothrsis that SFB is the pollen S gene. This hypothesis predicts involvement of the ubiquitin/26S proteasome proteolytic pathway in the RNase-based SI system.
キーワード
self-incompatibility
S-RNase
SFB
F-Box protein
ubiquitin proteasome proteolytic pathway
発行日
2005-02-01
出版物タイトル
岡山大学農学部学術報告
出版物タイトル(別表記)
Scientific reports of the Faculty of Agriculture, Okayama University
94巻
1号
出版者
岡山大学農学部
出版者(別表記)
Faculty of Agriculture,Okayama University
開始ページ
85
終了ページ
90
ISSN
0474-0254
NCID
AN00033029
資料タイプ
紀要論文
言語
Japanese
論文のバージョン
publisher
査読
無し
Eprints Journal Name
srfa