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ID 48099
フルテキストURL
タイトル(別表記)
Assay of GBM antigen in urine and serum with an anti-human renal monoclonal antibody
著者
味埜 泰明 岡山大学第三内科
抄録
Using a monoclonal antibody (Mab-G3) recognizing glomerular basement membrane (GBM), we assayed GBM antigen (G3-Ag) in the urine and serum of renal disease patients by sandwich ELISA. The subjects included normal control (NOR), minimal change nephrotic syndrome (MCNS), IgA nephropathy (IgA), membranous nephropathy (MN), membranoproliferative glomerulonephritis (MPGN) and chronic renal failure (CRF). The urine and serum was used as the material. With urinary G3-Ag, there were no statistically significant differences among the NOR, MCNS, IgA, MN, MPGN and CRF groups. Although no correlation was observed with proteinuria, hematuria, serum creatinine, serum β2 microglobulin and urinary NAG, urinary G3-Ag showed a significant (p<0.05) increase in excretion in the group of progressive CRF patients with s-Cr more than 1.0 mg/dl/month as compared to the stationary CRF group with s-Cr<1.0 mg/dl/month. Serum G3-Ag showed lower values in almost all cases, and there were no significant differences among the renal disease groups. The above findings led us to believe that the assay of urinary G3-Ag was useful in determining the degree of GBM disorder. It was also presumed that assay of renal antigens in urine and serum with the respective anti-human renal monoclonal antibodies could be a new tool in diagnosing renal diseases.
キーワード
monoclonal antibody
sandwich ELISA
GBM antigen
renal disease
発行日
1990
出版物タイトル
日本腎臓学会誌
出版物タイトル(別表記)
The Japanese Journal of Nephrology
32巻
4号
出版者
社団法人日本腎臓学会
出版者(別表記)
Japanese Society of Nephrology
開始ページ
365
終了ページ
372
ISSN
0385-2385
NCID
AN10131749
資料タイプ
学術雑誌論文
オフィシャル URL
http://joi.jlc.jst.go.jp/JST.Journalarchive/jpnjnephrol1959/32.365
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/6267
言語
Japanese
著作権者
Copyright© 社団法人日本腎臓学会
査読
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