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ID 58258
フルテキストURL
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著者
Kojima, Keiichi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University,
Shibukawa, Atsushi Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University,
Sudo, Yuki Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University ORCID Kaken ID researchmap
抄録
Microbial rhodopsins, a photoactive membrane protein family, serve as fundamental tools for optogenetics, an innovative technology for controlling biological activities with light. Microbial rhodopsins are widely distributed in nature and have a wide variety of biological functions. Regardless of the many different known types of microbial rhodopsins, only a few of them have been used in optogenetics to control neural activity to understand neural networks. The efforts of our group have been aimed at identifying and characterizing novel rhodopsins from nature and also at engineering novel variant rhodopsins by rational design. On the basis of the molecular and functional characteristics of those novel rhodopsins, we have proposed new rhodopsin-based optogenetics tools to control not only neural activities but also "non-neural" activities. In this Perspective, we introduce the achievements and summarize future challenges in creating optogenetics tools using rhodopsins. The implementation of optogenetics deep inside an in vivo brain is the well-known challenge for existing rhodopsins. As a perspective to address this challenge, we introduce innovative optical illumination techniques using wavefront shaping that can reinforce the low light sensitivity of the rhodopsins and realize deep-brain optogenetics. The applications of our optogenetics tools could be extended to manipulate non-neural biological activities such as gene expression, apoptosis, energy production, and muscle contraction. We also discuss the potentially unlimited biotechnological applications of microbial rhodopsins in the future such as in photovoltaic devices and in drug delivery systems. We believe that advances in the field will greatly expand the potential uses of microbial rhodopsins as optical tools.
発行日
2019-12-09
出版物タイトル
Biochemistry
59巻
3号
出版者
American Chemical Society
開始ページ
218
終了ページ
229
ISSN
0006-2960
NCID
AA00564599
資料タイプ
学術雑誌論文
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1021/acs.biochem.9b00768
Citation
Biochemistry 2020, 59, 3, 218-229 Publication Date:December 9, 2019 https://doi.org/10.1021/acs.biochem.9b00768
助成機関名
文部科学省