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ID 50676
フルテキストURL
著者
Akazawa, Y. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Kubo, M. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Zhang, R. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Matsumoto, K. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Yan, F. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Setiawan, H. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Takahashi, H. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
Fujikura, Y. Oita Univ, Fac Med, Dept Mol Anat
Ogino, K. Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Publ Hlth
抄録
Nitric oxide (NO) is produced from the conversion of L-arginine by NO synthase (NOS) and regulates a variety of processes in the gastrointestinal tract. Considering the increased activity of arginase in colitis tissue, it is speculated that arginase could inhibit NO synthesis by competing for the same L-arginine substrate, resulting in the exacerbation of colitis. We examined the role of arginase and its relationship to NO metabolism in dextran sulfate sodium (DSS)-induced colitis. Experimental colitis was induced in mice by administration of 2.5% DSS in drinking water for 8 days. Treatment for arginase inhibition was done by once daily intraperitoneal injection of N-omega-hydroxy-norarginine (nor-NOHA). On day 8, we evaluated clinical parameters (body weight, disease activity index, and colon length), histological features, the activity and expression of arginase, L-arginine content, the expression of NO synthase (NOS), and the concentration of NO end-product (NOx: nitrite + nitrate). Administration of nor-NOHA improved the worsened clinical parameters and histological features in DSS-induced colitis. Treatment with nor-NOHA attenuated the increased activity of arginase, upregulation of arginase. at both mRNA and protein levels, and decreased the content of L-arginine in colonic tissue in the DSS-treated mice. Conversely, despite the decreased expression of NOS2 mRNA, the decreased concentration of NOx in colonic tissues was restored to almost normal levels. The consumption of L-arginine by arginase could lead to decreased production of NO from NOS, contributing to the pathogenesis of the colonic inflammation; thus, arginase inhibition might be effective for improving colitis.
キーワード
nitric oxide
N-omega-hydroxy-nor-L-arginine
L-arginine
NOx
dextran sulfate sodium
発行日
2013-05
出版物タイトル
Free Radical Research
47巻
3号
出版者
Informa Healthcare
開始ページ
137
終了ページ
145
ISSN
1071-5762
資料タイプ
学術雑誌論文
オフィシャル URL
http://dx.doi.org/10.3109/10715762.2012.756980
関連URL
http://ousar.lib.okayama-u.ac.jp/metadata/50675
言語
English
OAI-PMH Set
岡山大学
論文のバージョン
author
査読
有り
DOI
Web of Sience KeyUT