フルテキストURL J_Pharm_Sci_130_1_24.pdf
著者 Tangsucharit, Panot| Takatori, Shingo| Zamami, Yoshito| Goda, Mitsuhiro| Pakdeechote, Poungrat| Kawasaki, Hiromu| Takayama, Fusako|
抄録 The present study investigated pharmacological characterizations of muscarinic acetylcholine receptor (AChR) subtypes involving ACh-induced endothelium-independent vasodilatation in rat mesenteric arteries. Changes in perfusion pressure to periarterial nerve stimulation and ACh were measured before and after the perfusion of Krebs solution containing muscarinic receptor antagonists. Distributions of muscarinic AChR subtypes in mesenteric arteries with an intact endothelium were studied using Western blotting. The expression level of M1 and M3 was significantly greater than that of M2. Endothelium removal significantly decreased expression levels of M2 and M3, but not M1. In perfused mesenteric vascular beds with intact endothelium and active tone, exogenous ACh (1, 10, and 100 nmol) produced concentration-dependent and long-lasting vasodilatations. In endothelium-denuded preparations, relaxation to ACh (1 nmol) disappeared, but ACh at 10 and 100 nmol caused long-lasting vasodilatations, which were markedly blocked by the treatment of pirenzepine (M1 antagonist) or 4-DAMP (M1 and M3 antagonist) plus hexamethonium (nicotinic AChR antagonist), but not methoctramine (M2 and M4 antagonist). These results suggest that muscarinic AChR subtypes, mainly M1, distribute throughout the rat mesenteric arteries, and that activation of M1 and/or M3 which may be located on CGRPergic nerves releases CGRP, causing an endothelium-independent vasodilatation.
キーワード Acetylcholine-induced vasodilatation CGRPergic nerves Muscarinic receptor subtypes Rat mesenteric arteries
発行日 2016-01
出版物タイトル Journal of Pharmacological Sciences
130巻
1号
出版者 Japanese Pharmacological Society
開始ページ 24
終了ページ 32
ISSN 1347-8613
NCID AA11806667
資料タイプ 学術雑誌論文
言語 English
OAI-PMH Set 岡山大学
著作権者 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
論文のバージョン publisher
PubMed ID 26825997
DOI 10.1016/j.jphs.2015.12.005
Web of Sience KeyUT 000369700500004
関連URL https://doi.org/10.1016/j.jphs.2015.12.005