| フルテキストURL |
viruses-09-00371.pdf
|
| 著者 |
Ogawa, Hirohito|
Kajihara, Masahiro|
Nao, Naganori|
Shigeno, Asako|
Fujikura, Daisuke|
Hang’ombe, Bernard M.|
Mweene, Aaron S.|
Mutemwa, Alisheke|
Squarre, David|
Yamada, Masao|
Higashi, Hideaki|
Sawa, Hirofumi|
Takada, Ayato|
|
| 抄録 |
Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name "Bat mastadenovirus H". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.
|
| キーワード |
Eidolon helvum
Zambia
adenovirus
bat
|
| 発行日 |
2017-12-04
|
| 出版物タイトル |
Viruses
|
| 巻 |
9巻
|
| 号 |
12号
|
| 出版者 |
MDPI
|
| 開始ページ |
371
|
| ISSN |
1999-4915
|
| 資料タイプ |
学術雑誌論文
|
| 言語 |
English
|
| OAI-PMH Set |
岡山大学
|
| 著作権者 |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
|
| 論文のバージョン |
publisher
|
| PubMed ID |
29207524
|
| DOI |
10.3390/v9120371
|
| 関連URL |
isVersionOf https://doi.org/10.3390/v9120371
|
